Waters Technologies Corporation

Robert Birdsall

Matthew A Lauber

Jennifer M Nguyen

Henry Y Shion

Ximo Zhang

Nilini Ranbaduge

Moon Chul Jung

Thomas H Walter

Xiaoxiao Liu

Erin E Chambers

Christopher John Hughes

Ying Qing Yu

Melvin Blaze Muttikal Thomas

Yun Wang Alelyunas

Mary Elizabeth Lame

Paula Orens

Jacob Kellett

Caitlin Dunning

David Dao

The present disclosure relates to a sensitive, multidimensional chromatography method for extraction, detection, and quantification of non-conjugated cytotoxic agents and associated linker molecules used in cysteine based antibody-drug-conjugate production.

Multidimensional chromatography method for analysis of antibody-drug conjugates

The present technology relates to a method of analyzing a sample including an analyte. The method includes injecting the sample including the analyte into a mobile phase. The mobile phase includes a metal chelator additive having a concentration between about 1 ppm to about 10 ppm. The method also includes separating the analyte using liquid chromatography and analyzing the analyte using a mass spectrometer, an ultra-violet detector, or a combination thereof.

RPLC-based peptide mapping chromatographic performance using metal chelators as mobile phase additives

The present disclosure relates to a method of analyzing a sample comprising a hydrophobic molecule. The method includes preparing an aqueous solution comprising the sample. The method also includes placing the solution in contact with a polypropylene substrate having a deactivated surface that reduces adsorption of the hydrophobic molecule relative to a polypropylene substrate that has not been deactivated. The method also includes analyzing the sample.

Methods of use for low-bind polypropylene plates and vials

The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. A mobile phase is flowed through a chromatography column. The mobile phase includes about 0.005% (v/v) to about 2.50% (v/v) difluoroacetic acid and less than about 100 ppb of any individual impurity, especially metal impurities. A sample including the analyte is injected into the mobile phase. The analyte is separated from the sample.

Difluoroacetic acid ion pairing reagent for high sensitivity, high resolution LC-MS of biomolecules and small molecules

The present disclosure relates to the determination of analytes in a sample using chromatography. The present disclosure provides methods of separating an analyte from a sample. A mobile phase is flowed through a chromatography column. The mobile phase includes about 0.005% (v/v) to about 0.20% (v/v) difluoroacetic acid and less than about 100 ppb of any individual metal impurity. A sample including the analyte is injected into the mobile phase. The analyte is separated from the sample.

Difluoroacetic acid ion pairing reagent for high sensitivity, high resolution LC-MS of biomolecules

The present technology provides methods for increasing sensitivity of detection and/or quantification of a negatively charged analyte, e.g., an oligonucleotide, using an analytical system that comprises liquid chromatography and mass spectrometry. The methods comprise passing an acidic solution through the analytical system, i.e., through a fluidic path from the mobile phase reservoir to the detector to remove or displace, at least in part, metal ions adsorbed to charged sites in the fluidic path.

Methods for increasing sensitivity of detection and/or quantification of negatively charged analytes

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Southborough, MA, United States of America

Robert Birdsall