North Carolina State University

Pharmacia Biotech Inc.

Pharmacia P-l Biochemicals Inc.

Robert A Luhm

James F Jolly

Robert M Kelly

Phillip P Franciskovich

Eve Y Ting

William A Riedl

Olga B d'Hennezel

Susan G Frackman

Guy D Duffaud

A preparation of a purified DNA polymerase substantially identical to native DNA polymerase isolated from Thermococcus barossii is disclosed. In a preferable form of the present invention, the DNA polymerase is approximately 68 kDa in size, retains at least 90% activity after 8 hours of incubation at 95.degree. C. and retains between 60% and 80% proof-reading ability when catalyzing a polymerization reaction with a primer with a 3 base pair mismatch.

Purified Thermococcus barossii DNA polymerase

A bacterial preparation capable of packaging phage .lambda. DNA is disclosed. This preparation is in lyophilized form and is stable at ambient temperature. In a preferred form, the preparation contains an over-expressed terminase protein, is prepared from the bacterial strain E. coli Cla [.lambda. c/857 Sam7 .DELTA.(cos-Nu1-A)::Kn.sup.r ]/.lambda. pTER and is capable of a packaging efficiency of at least 1.times.10.sup.8 pfu/.mu.g wild type .lambda. DNA. The present invention is also a method of creating a phage .lambda. DNA packaging extract comprising the steps of preparing a bacterial extract capable of packaging phage .lambda. and lyophilizing said extract.

In vitro phage lambda DNA packaging system

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Mequon, WI, United States of America

Robert A Luhm