University of Washington

Robert A Langan

David Baker

Scott Boyken

Marc J Lajoie

Alfredo Quijano Rubio

Walter Novak

Zibo Chen

Sherry Bermeo

Chunfu Xu

Disclosed are protein switches that can sequester bioactive peptides and/or binding domains, holding them in an inactive ('off') state, until combined with a second designed polypeptide called the key, which induces a conformational change that activates (“on”) the bioactive peptide or binding domain, components of such protein switches, and their use.

De novo design of protein switches

Disclosed herein are designed heterodimer proteins, monomeric polypeptides capable of forming heterodimer proteins, protein scaffolds including such polypeptides, and methods for using the heterodimer proteins and subunit polypeptides for designing logic gates.

Orthogonal protein heterodimers

Methods and apparatus for identifying and screening hydrogen bond networks are provided. A computing device can determine a search space for hydrogen bond networks related to one or more molecules, where the search space can include a plurality of energy terms related to a plurality of residues related to the hydrogen bond networks. The computing device can search the search space to identify one or more hydrogen bond networks based on the plurality of energy terms. The computing device can screen the identified hydrogen bond networks to identity one or more screened hydrogen bond networks based on scores for the identified hydrogen bond networks. An output can be generated that is related to the one or more screened hydrogen bond networks. Also provided are polypeptides that can form homo-oligomers with modular hydrogen bond network-mediated specificity.

Polypeptdes capable of forming homo-oligomers with modular hydrogen bond network-mediated specificity and their design

Disclosed are protein switches that can sequester bioactive peptides and/or binding domains, holding them in an inactive ('off') state, until combined with a second designed polypeptide called the key, which induces a conformational change that activates ('on') the bioactive peptide or binding domain, components of such protein switches, and their use.

Growing community of inventors

Seattle, WA, United States of America

Robert A Langan