Lumigen, Inc.

Beckman Coulter, Inc.

Tropix, Inc.

Robert A Eickholt

Hashem Akhavan-Tafti

Richard S Handley

Kenneth S Lauwers

Zahra Arghavani

Renuka De Silva

Ravinder K Gupta

Lakshmi Sireesha Kaanumalle

Guoping Wang

Wenhua Xie

Jaya S Koti

Mark D Sandison

David L Schumm

Khaledur S Rashid

Lakshmi S Kaanumalle

Alkaline phosphatase chemiluminescent substrate formulations are provided exhibiting rapid incubation periods and improved stability for use in immunoassays.

Chemiluminescent substrates

The present invention provides fluorescent dyes that are based on firefly luciferin structure. These dyes are optimally excited at shorter wavelengths and have Stokes shift of at least 50 nm. The fluorescent dyes of the invention are useful for preparation of dye-conjugates, which can be used in detection of an analyte in a sample.

Fluorescent dyes and uses thereof

Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig-OH or Sig-Ois produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.

Compositions for chemiluminescent detection of hydrogen peroxide

Signalling compounds for use in methods of detecting hydrogen peroxide

Methods and compound useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula: is reacted with peroxide. Sig is a non-polymeric organic group, B is a boron atom, and each R is independently selected from hydrogen, alkyl and aryl groups and can be joined together as a straight or branched alkylene chain forming a ring or as an aromatic ring. A detectable product compound of the formula Sig&#x2014;OH or Sig&#x2014;Ois produced and detected by measuring color, absorbance, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The methods can be used as a detectable signal in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.

Signalling compounds and methods for detecting hydrogen peroxide

Compounds useful for detecting a source of hydrogen peroxide are disclosed wherein a signalling compound of the formula:is reacted with peroxide. Sig is an aromatic or heteroaromatic ring group, B is a boron atom, and Rand Rare independently selected from hydrogen and lower alkyl groups and can be joined together as a straight or branched alkylene chain forming a five or six-membered ring. A detectable product compound of the formula Sig-OH Is produced and detected by measuring color, fluorescence, chemiluminescence, or bioluminescence. The signalling compound itself does not possess the detectable property or does so only to a very weak degree. The compounds can be used for detection in assays for peroxide or peroxide-producing enzymes and in assays employing enzyme-labeled specific binding pairs.

Synthetic processes and intermediates are disclosed for the preparation of N-arylacridancarboxylic acid derivatives. The derivatives are esters, thioesters, amides and sulfonimides. A key feature of the processes is the preparation of N-aryl substituted intermediates by formation of a bond between the nitrogen atom of the acridan ring and a carbon atom of another aromatic or heteroaromatic ring compound. The arylation reaction is catalyzed by a palladium catalyst. The N-arylacridancarboxylic acid derivatives are useful in methods for producing light and in assays for peroxidase enzymes and enzyme inhibitors and in assays employing enzyme-labeled specific binding pairs.

Processing and synthetic intermediates for preparing N-arylacridancarboxylic acid derivatives

Process for the preparation of 1,2-dioxetane compounds and novel

Vinyl sulfide compounds and a process for their preparation

A chemiluminescent assay method and compositions are described which use a haloalkoxy group-substituted dioxetane which is deprotected by a hydrolytic enzyme to undergo a chemiluminescent reaction. Chemiluminescent 1,2-dioxetane compounds substituted on the dioxetane ring with a haloalkoxy group which can be triggered by a reagent to generate light are disclosed. Haloalkoxy group-substituted dioxetanes are useful for the detection of triggering agents including enzymes. The enzyme may be present alone or linked to a member of a specific binding pair in an immunoassay, DNA probe assay or other assay where the enzyme is bound to a reporter molecule.

Growing community of inventors

Troy, MI, United States of America

Robert A Eickholt