Discovery Partners International, Inc.

Nexus Biosystems, Inc.

Rob Neeper

John E Lillig

John F Bridwell

Robert K Levin

Rhett L Affleck

Thuc Nguyen

Allan Childers

The automated sample-on-solid-support processing system of the present invention comprises a computer-based control unit and a main unit comprising a variable-speed centrifuge having an openable vacuum-tight chamber and a centrifuge rotor with a plurality of multi-sample holding positions, a liquid solvent supply subsystem which feeds solvent to a plurality of dispensing stations in the centrifuge chamber, a temperature control subsystem, and a vacuum subsystem. A sample/collection container includes a plurality of wells, each for separating a sample from its solid support when solvent is dispensed into the wells and the centrifuge is activated at a low speed. Operation of the centrifuge at high speed concentrates the cleaved sample in collection wells. In the preferred embodiment bar code reader or other identification means, preferably a non-contact reader, can be included in the chamber to allow sample carriers to be identified.

Automated method for treatment of samples on solid supports

System and method for treatment of samples on solid supports

An automated sample-on-solid-support processing system includes a treatment solution supply subsystem which feeds solvent to a dispenser to dispense a measured amount of treatment solution to each well of a multi-well plate. The dispenser measures the solution within a fill container having a plurality of reservoir wells corresponding to the number of wells in the multi-well plate. The fill container is contained within a reservoir chamber that is filled with an excess of solution. Each reservoir well is surrounded by a plurality of bores which drains excess solution from the top of the fill container so that a precise amount of solution is left in the reservoir wells when solution is drained out of the reservoir chamber. Gas pressure is introduced into the reservoir chamber to force the solution out of the reservoir well through a bundle of tubes, with one tube per well, and into the wells of the multi-well plate.

System and method for dispensing solution to a multi-well container

A sample/collection container includes a plurality of wells, each for separating a sample from its solid support when solvent is dispensed into the wells and the centrifuge is activated at a low speed. Operation of the centrifuge at high speed concentrates the cleaved sample in collection wells.

Container and method for high volume treatment of samples on solid supports

The resin dispenser comprises a support frame, a probe with an adjustable loading volume mounted on the support frame and connected to a vacuum source, a hopper for containing a resin slurry, a holder for retaining one or more containers to be loaded at a loading position, a translation means for moving the probe from the hopper to the container, a fluid circulation pump and an air pump for circulating fluid in the hopper, and at least one control device. The hopper includes a means for continuous circulation and replenishment of solvent with a large reserve of resin, and has a small volume from which the resin is extracted. The method for dispensing comprises drawing resin from a dispenser trough in the hopper into the loading volume using a vacuum, moving the probe to the loading position, then activating an air actuator which expels a resin pellet from the probe into the container by initiating movement of the inner and outer tubes that define the loading volume.

Growing community of inventors

Lakeside, CA, United States of America

Rob Neeper