Butamax Advanced Biofuels LLC

Ritu Bhalla

Ranjan Patnaik

Gopal K Chotani

Caroline M Peres

Daniel P O'Keefe

Mark J Nelson

Jean-Francois Tomb

Michael Dauner

Jahnavi Chandra Prasad

Steven D Doig

Kakasaheb Suresh Konde

Vasantha Nagarajan

Arthur Leo Kruckeberg

Wonchul Suh

Bhaskar Bhadra

Provided herein are processes for producing an improved culture of cells comprising an engineered butanol biosynthetic pathway. The processes comprise (a) providing a cell culture of recombinant microorganisms comprising an engineered butanol biosynthetic pathway, wherein the engineered butanol biosynthetic pathway is minimal or not activated; and (b) growing the culture of recombinant microorganisms under adaptive conditions whereby pathway activation is increased to produce an improved cell culture and whereby the improved cell culture is capable of continuing to grow in fermentation.

Partial adaption for butanol production

Provided herein are glycerol-3-phosphate dehydrogenase (GPD) enzymes with increased Kfor NADH and GPD enzymes with substantially the same affinity for NADH and NADPH and/or are feedback inhibited by glycerol-3-phosphate. Also provided herein are recombinant microorganisms comprising a heterologous gene encoding GPD and a deletion or disruption in an endogenous gene encoding GPD. Also provided are recombinant microorganisms comprising a heterologous gene encoding GPD and a butanol biosynthetic pathway. Further provided are methods of producing butanol comprising providing the recombinant microorganisms described herein and contacting the recombinant microorganism with at least one fermentable carbon substrate under conditions wherein butanol is produced.

Glycerol 3-phosphate dehydrogenase for butanol production

The invention relates to the fields of industrial microbiology and alcohol production. The invention also relates to the development of a microorganism capable of producing fermentation products via an engineered pathway, and uses of the microorganism. The invention also relates to the methods to improve cell viability and productivity and the use of recycling and acid washing to increase the yield of fermentation products.

Fermentative production of alcohols

Partial adaptation for butanol production

Glycerol 3- phosphate dehydrogenase for butanol production

Growing community of inventors

Tirmulgherry, India

Ritu Bhalla