Kabushiki Kaisha Toyota Chuo Kenkyusho

Toyota Jidosha Kabushiki Kaisha

Ritsuko Yogo

Nobuhiko Muramoto

Hiroki Sugimoto

Norihiro Mitsukawa

Tomoko Tanaka

Chikara Ohto

Satoshi Kondo

Hidenori Tanaka

Satoshi Katahira

Akinori Ikeuchi

Risa Nakamura

Kazufumi Tabata

A method of inducing genetic recombination, including: allowing a protein having DNA double-stranded cleavage activity to act in cells of a eukaryotic organism which is a polyploidy inherently possessed by a eukaryotic organism. In eukaryotic organisms, various genetic recombination generates new genome set composition. This is done to obtain a population of eukaryotic organisms that hold the modified genomic set.

Method of inducing genetic recombination, and use therefor

Provided is a genome shuffling technology suited to creating a useful next-generation plant body. Genome shuffling is performed using a polypeptide comprising a first polypeptide region for double-stranded DNA breakage activity and a second polypeptide region for intertissue migration activity.

Polypeptide for genome shuffling in plants, and use therefor

This invention is intended to increase biomass production through suppression of expression of a gene functioning in relation to intracellular vesicular transport or inhibition of functions of a protein encoded by such gene. To this end, expression of a gene encoding the coatomer adapter zeta subunit is suppressed, the coatomer adapter zeta subunit is inhibited, expression of a gene encoding the clathrin adaptor small (sigma) subunit is suppressed, or the clathrin adaptor small (sigma) subunit is inhibited.

Plant in which expression of a clathrin and/or coatomer subunit is suppressed or inhibited, a method for increasing the production of plant biomass and a method for producing a plant

A method for producing a plant includes growing a plant from plant cells in which a promoter and a coding sequence under the control of the promoter have been artificially introduced; and selecting the plant in which an amount of phloem tissue is increased 2.0 times or more in comparison with a wild type plant of the same species. The promoter is a promoter for a gene encoding a protein having at least 60% identity with the amino acid sequence of tracheary element differentiation inhibitory factor (TDIF) receptor (TDR) of. The coding sequence encodes either (i) a protein having three common sequences composed of the amino acid sequences of SEQ ID NOS: 1 to 3 in this order starting from the N-terminal side, or (ii) a protein having 95% or more identity with the amino acid sequence of SEQ ID NO:5.

Plant suitable for increasing production of phloem tissue and use of same

Growing community of inventors

Nagakute, Japan

Ritsuko Yogo