Shiseido Company, Limited

The General Hospital Corporation

Ritsuko Ehama

Kazuo Watabe

Satoshi Amano

Jiro Kishimoto

Robert E Burgeson

Masahiro Tajima

Takayuki Arai

Tsutomu Soma

Toshihiko Kaminuma

Toshio Nishiyama

Kiichiro Yano

Ritsuro Ideta

Takashi Yoshimoto

Takaaki Baba

Shoji Fukushima

Robert Eugene Burgeson

The present invention provides a method for preparing a hair dermal papilla cell preparation comprising preparing a cell suspension by removing epidermal tissue from skin tissue and subjecting the resulting dermal tissue fraction to collagenase treatment, and cyropreserving the cell suspension to kill the follicular epidermal cells. The present invention also provides a composition for regenerating hair follicles comprising hair dermal papilla cell and epidermal cells, wherein the ratio of the number of hair dermal papilla cell to the number of epidermal cells is from 1:10 to 10:1.

Preparation method of a hair dermal papilla cell preparation, composition and method for regenerating hair follicles, and animal having regenerated hair follicles

The invention provides methods of evaluating a treatment for its effect on skin. The invention also provides non-human transgenic animals, e.g., mice, having a reporter gene coupled to a skin-metabolism promoter.

Method for evaluating a compound for its effect on skin

A prolonged releasing pharmaceutical preparation is provided carrying a physiologically active substance, particularly, calcitonin gene-related peptide (CGRP) or a maxadilan (MAX). This pharmaceutical preparation can attain the expected effects by incorporating the physiologically active substance into a combination, as carriers for the physiologically active substance, of a cellulosic polymer and at least one auxiliary component selected from the group consisting of fats and oils, waxes, fatty acids, saccharides and polyacrylate ester derivatives. The pharmaceutical preparation can conveniently be used, in living bodies, particularly as an intrathecal implantation-type preparation.

Physiologically active substance-prolonged releasing-type pharmaceutical

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Yokohama, Japan

Ritsuko Ehama