University of Minnesota

Rita Perlingeiro

Daniel F Voytas

Michael Kyba

Fabrizio Rinaldi

Ricardo Mondragon Gonzalez

Sridhar Selvaraj

A method to increase the efficiency of myotube generation and maturation from pluripotent stem cells comprising: (a) differentiating pluripotent stem cells to myogenic progenitors; and (b) terminally differentiating said myogenic progenitors from (a) into myotubes in the presence of at least one gamma secretase inhibitor, wherein myotube generation is increased in the presence of at least one gamma secretase inhibitor, as compared to differentiation in the absence of gamma secretase inhibitors.

Enhanced differentiation and maturation of pluripotent stem cell-derived myogenic cells

Provided herein is a general method of correcting a gene, for example a gene with many known mutations, such as a gene with mutations in many different exons which could vary from subject to subject (e.g., patient), as well as a set of tools (TALENs and gene targeting vectors) to accomplish such method.

Growing community of inventors

Minneapolis, MN, United States of America

Rita Perlingeiro