Dupont Nutrition Biosciences Aps

Rikke H Lorentzen

Richard Ray Bott

Marguerite A Cervin

Jørn Borch Søe

Charlotte Horsmans Poulsen

Jørn Dalgaard Mikkelsen

Janne Brunstedt

Charlotte Refdahl Thoudahl

Morten Krog Larsen

Lone Broend Miller

Charlotte Johansen Vedel

Birgitte Ø Wittschieben

Lene B Jensen

Jens Frisbæk Sorensen

The present invention provides a method for preparing a variant lipid acyltransferase enzyme by expressing a nucleotide sequence encoding a lipid acyltransferase which may comprise at least one modification at a position(s) which corresponds in the encoded amino acid sequence to an amino acid(s) located in a) the canyon region of the enzyme (i.e. preferably amino acid residues 31, 27, 85, 86, 119, and 120); and/or b) insertion site 1 (i.e. amino acid residues 22-36) and/or c) insertion site 2 (i.e. amino acid residues 74-88), wherein the canyon region, insertion site 1 and/or insertion site 2 are defined as that region which when aligned based on primary or tertiary structure corresponds to the canyon region, insertion site 1 or insertion site 2 (or the corresponding amino acid residues taught above) of the enzyme shown herein as SEQ ID No. 16 or 6 in a host organism.

Lipid acyltransferase proteins and methods of making them

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Randers, Denmark

Rikke H Lorentzen