E.i. Dupont De Nemours and Company

Butamax Advanced Biofuels LLC

Butamax (Tm) Advanced Biofuels LLC

International N&h Denmark Aps

Rick Weizhang Ye

Dennis Flint

Brian James Paul

Kristen J Kelly

James M Odom

Mattheos Koffas

Kelley C Norton

Steven Cary Rothman

Laura Anne Bedzyk

Tao Wang

Zhongqiang Chen

Lori Ann Maggio-Hall

Stephen K Picataggio

Larry Cameron Anthony

Jean-Francois Tomb

Wonchul Suh

John E Seip

Raymond E Jackson

Hao Ni

Deana J Dicosimo

Quinn Qun Zhu

Vasantha Nagarajan

Richard C Ebersole

Siqun Wang

Tina K Van Dyk

Pamela L Sharpe

Lixuan Lisa Huang

Robert A Larossa

Qiong Wang

Michael W Bostick

Stephen Varkey

Daniel R Demarco

John Thomas Gannon

Alexander D Kopatsis

Henry Yao

Andreas J Schenzle

Daniel DeMarco

Amanda Chan

Victoria Vallejo Kelly

Claus Lang

Julia Yager

J Martin Odom

Kristen Janet Stead

Provided herein, inter alia, are compositions of short chain fatty acid (SCFA)-producing microorganisms and methods of making and using the same to inhibit pathogenic bacterial populations in the gastrointestinal tracts of an animal and additionally promote improvement of one or more metrics in an animal, such as increased bodyweight gain, decreased feed conversion ratio (FCR), improved gut barrier integrity, reduced mortality, reduced pathogen infection, and reduced pathogen shedding in feces.

Compositions for gut health

The present invention is related to a recombinant host cell, in particular a yeast cell, comprising a dihydroxy-acid dehydratase polypeptide. The invention is also related to a recombinant host cell having increased specific activity of the dihydroxy-acid dehydratase polypeptide as a result of increased expression of the polypeptide, modulation of the Fe&#x2014;S cluster biosynthesis of the cell, or a combination thereof. The present invention also includes methods of using the host cells, as well as, methods for identifying polypeptides that increase the flux in an Fe&#x2014;S cluster biosynthesis pathway in a host cell.

Activity of Fe&#x2014;S cluster requiring proteins

Methods of screening for dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed, along with DHAD variants identified by these methods. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.

DHAD variants and methods of screening

Dihydroxy-acid dehydratase (DHAD) variants that display increased DHAD activity are disclosed. Such enzymes can result in increased production of compounds from DHAD requiring biosynthetic pathways. Also disclosed are isolated nucleic acids encoding the DHAD variants, recombinant host cells comprising the isolated nucleic acid molecules, and methods of producing butanol.

DHAD variants for butanol production

Activity of Fe-S cluster requiring proteins

Specific polypeptides were identified as bacterial xylose isomerases that are able to provide xylose isomerase activity in yeast cells. The xylose isomerase activity can complete a xylose utilization pathway so that yeast can use xylose in fermentation, such as xylose in biomass hydrolysate.

Bacterial xylose isomerases active in yeast cells

A group of bacterial dihydroxy-acid dehydratases having a [2Fe-2S] cluster was discovered. Bacterial [2Fe-2S] DHADs were expressed as heterologous proteins in bacteria and yeast cells, providing DHAD activity for conversion of 2,3-dihydroxyisovalerate to &#x3b1;-ketoisovalerate or 2,3-dihydroxymethylvalerate to &#x3b1;-ketomethylvalerate. Isobutanol and other compounds may be synthesized in pathways that include bacterial [2Fe-2S] DHAD activity.

Identification and use of bacterial [2Fe-2S] dihydroxy-acid dehydratases

Polypeptides were identified among translated coding sequences from a metagenomic cow rumen database, that were shown to provide xylose isomerase activity in yeast cells. The xylose isomerase activity can complete a xylose utilization pathway so that yeast can use xylose in fermentation, such as xylose in biomass hydrolysate.

Cow rumen xylose isomerases active in yeast cells

Engineered strains of the oleaginous yeastcapable of producing carotenoids (e.g., &#x3b2;-carotene, lycopene, lutein, zeaxanthin, canthaxanthin, astaxanthin) are provided. The strains may also be engineered to co-produce at least one &#x3c9;-3/&#x3c9;-6 polyunsaturated fatty acid and/or at least one additional antioxidant. Methods of using the carotenoid products obtained (e.g., biomass and/or pigmented oils) in food and feed applications are also provided.

Carotenoid production in a recombinant oleaginous yeast

Yeast cells with modified expression of certain enzyme activities in the mitochondria are described for isobutanol production. Modifications described provide an isobutanol biosynthesis pathway in the yeast mitochondria.

Production of isobutanol in yeast mitochondria

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Hockessin, DE, United States of America

Rick Weizhang Ye