The Scripps Research Institute

International Aids Vaccine Initiative

Mabtech Ab

Richard Wyatt

Viktoriya Dubrovskaya

Francisco Javier Guenaga

Yu Feng

Andrew B Ward

Staffan Paulie

Javier Guenaga

Karen Tran

Shailendra Kumar

Jidnyasa Ingale

Natalia De Val Alda

Pia Dosenovic

Shailendra Kumar Sharma

Gunilla Karlsson Hedestam

The present application relates to novel HIV-1 envelope glycoproteins which may be utilized as an HIV-1 vaccine immunogens, antigens for crystallization and for the identification of broad neutralizing antibodies. The present invention encompasses the preparation and purification of immunogenic compositions which are formulated into the vaccines of the present invention.

N-glycan deleted HIV-1 envelope glycoprotein trimers

An improved assay is described where a surface is provided with immobilized anti-Ig antibodies rather than antigen and where specific antibody-secreting cells (ASC) are detected using soluble antigen probes containing one of several possible labels. The method gives improved sensitivity with less background and is also more representative because antigen binding does not employ immobilized antigen. The assay is particularly effective for measuring antibody secreting cells against HIV, for determining whether an infection is acute as opposed to old or latent, for mapping epitopes and for measuring for ASCs against different antigens in the same reaction.

Antibody-secreting cell assay

Soluble HIV-1 envelope glycoprotein trimers

Growing community of inventors

La Jolla, CA, United States of America

Richard Wyatt