Eastman-kodak Company

Richard W Spayd

Sang H Kim

Tai-Wing Wu

David A Hilborn

Brent A Burdick

Disclosed herein is an assay useful for the quantitative determination of hemoglobin in whole blood. This assay utilizes a multizone element comprising a registration zone comprising particulate barium sulfate distributed in a 'blush' polymer, and a spreading zone having a void volume and an average pore size (e.g. greater than 5 microns) effective to accommodate whole blood. The element also contains an interactive composition capable of converting substantially all forms of hemoglobin into a detectable end product. One particularly useful interactive composition comprises a hemoglobin oxidizing agent (e.g. ferricyanide) and thiocyanate. Another useful interactive composition comprises an ionic surfactant, e.g. sodium octyl sulfate or sodium dodecyl sulfate.

Analytical element and its use in a whole blood hemoglobin assay

Novel elements for the analysis of liquids which contain interferents for an analyte assay are described. Interference is reduced in multizone elements by the presence of an interferent-removing zone between the point of introduction of the liquid and the assay-indicator zone wherein the interferent-removing zone comprises a discontinuous phase within a continuous phase. An interferent-removing material is present in the discontinuous phase and the continuous phase is permeable to the analyte, its products and/or the liquid being analyzed.

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Rochester, NY, United States of America

Richard W Spayd