Cephalon, Inc.

Richard W Scott

Andrew G Reaume

Robert Siman

Sheryl L Meyer

Karen Dorfman

Russell A Brierley

Stephen P Trusko

David S Howland

Eric K Hoffman

The present invention provides a gene-targeted, non-human mammal having a gene encoding a mutant protein product of a mutated FAD presenilin-1 (PS-1) gene, a human FAD Swedish mutation, and a humanized A&bgr; mutation, and generational offspring thereof and a gene-targeted, non-human mammal having a gene encoding a mutant protein product of a mutated FAD PS-1 gene and a human Swedish APP695 mutation, and generational offspring thereof, as well as methods of identifying compounds useful in treating Alzheimer's disease, and to methods of treating Alzheimer's disease.

Gene-targeted non-human mammal with human fad presenilin mutation and generational offspring

This invention discloses a gene-targeted non-human mammal and generational offspring with respect to the gene encoding a mutant protein product of a mutated presenilin 1 gene (PS-1); more specifically, a gene-targeted mouse with respect to the gene sequence encoding the mutant protein product of PS-1 which has been mutated to contain the human P264L mutation. Methods for screening chemical compounds using such mammals are also disclosed.

Gene-targeted non-human mammal with a human fad presenilin mutation and generational offspring

The present invention is directed to mammalian enzymatically active calpain produced in insect cells by recombinant means. Recombinant vectors and baculoviruses containing cDNA encoding the 80 kDa subunit, 30 kDa subunit, and both subunits are described. Methods for producing recombinant enzymatically active mammalian calpain are also described.

Recombinant enzymatically active calpain expressed in a baculovirus

Gene-targeted heterozygous and homozygous SOD-1 null non-human mammals, methods for producing them, and methods for use are described. Deletion vectors and gene-targeted cells are also described, as are methods for producing and using the same.

Gene-targeted non-human mammals deficient in the SOD-1 gene

Disclosed is non-human mouse homozygous for a targeted amyloid precursor protein-encoding gene comprising: (1) a human A.beta. peptide-encoding sequence in place of the native A.beta. peptide-encoding sequence; and (2) Swedish FAD mutations.

Gene-targeted mice with humanized A.beta. sequence and Swedish FAD

The invention generally features a novel recombinant DNA molecule useful for producing a heterologous protein, e.g., insulin-like growth factor-I, in yeast. The DNA molecule includes the following sequences joined in frame and in the following 5' to 3' progression: a sequence encoding a signal peptide of a yeast secreted protein; a sequence encoding at least 5 amino acids of the N-terminal region of a mature acid phosphatase protein, a sequence encoding a heterologous protein. Preferably the DNA molecule also encodes a spacer element that includes a proteolytic cleavage site.

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Wallingford, PA, United States of America

Richard W Scott