The State University of New York

The Penn State Research Foundation

Quadrant Biosciences Inc.

Richard Uhlig

Steven D Hicks

Frank A Middleton

Alexander Rajan

A method for evaluating or monitoring metabolic effects of exercise or injury by detecting and/or quantifying microR-NAs in saliva. Compositions and methods of treatment based on detection of microRNAs.

Method for monitoring metabolism before and after exercise or injury using salivary micro-RNAs

Provided are methods for detecting or diagnosing a traumatic brain injury or TBI by detecting concentration levels miRNAs associated with TBI in saliva. Methods for controlled and normalized comparisons of salivary miRNA concentration levels are further provided. Assay kits comprising salivary miRNAs, probes and/or primers for detecting salivary miRNAs are also provided.

Analysis and prediction of traumatic brain injury and concussion symptoms

This application provides methods to differentiate between subjects with autism spectrum disorder (ASD) and typically developing (TD) or developmentally delayed (DD) subjects using miRNA and/or microbiome levels detected in saliva samples and patient information. The method can be used to monitor the progress of ASD and guide its treatment. RNA-seq, qPCR, or other methods determine counts and abundance of miRNA or microbiomes. MicroRNA and/or microbiome sequencing data are refined by normalization to expression levels or abundance of time-invariant miRNAs and/or microbial RN As to control for time of sample collection or to compensate for circadian fluctuations in these levels. Multivariate logistic regression and nonlinear classification techniques are further used to select a panel of miRNAs and microbiomes that accurately differentiate between subjects with ASD, DD, and TD in subjects with an unknown ASD status. These panels of miRNAs and microbiomes may be developed into a RNA assay kit.

Growing community of inventors

Ithaca, NY, United States of America

Richard Uhlig