Pressure Biosciences, Inc.

Florida International University

Richard T Schumacher

Edmund Y Ting

Bruce McCord

Alexander Lazarev

Vera Gross

Deepthi V Nori

Nathan P Lawrence

Devices and methods for sample preparation via pressure cycling technology are disclosed. The device for sample preparation comprises a tube having an inner surface, a top, and a bottom, the tube configured to contain a sample at the bottom and to be received into a pressure chamber for sample preparation, a cap detachably connected to the top of the tube, and a tapered elongate member extending from the cap into the tube, the tapered elongate member configured to contact the inner surface of the tube and the sample in the bottom of the tube, wherein the tube is deformable such that in operation under pressure the tube is deformed against the tapered elongate member to promote disruption of the sample.

Sample preparation devices and methods

The subject invention provides a two-step protocol using pressure cycling technology (PCT) and alkaline lysis for differential extraction of mixtures. In a preferred embodiment the procedure is used in forensic DNA applications such as, for example, DNA testing in the case of rape.

Materials and methods for achieving differential lysis of mixtures with the aid of alkaline lysis and pressure cycling technology (PCT)

Growing community of inventors

South Easton, MA, United States of America

Richard T Schumacher