Applied Biosystems, LLC

Applera Corporation

Genapsys, Inc.

Life Technologies Corporation

Hitachi-high-technologies Corporation

Pe Corporation

Richard T Reel

Mark F Oldham

Eric S Nordman

David Cox

Linda G Lee

Sam L Woo

Hesaam Esfandyarpour

Cheryl Heiner

John S Shigeura

Frank Lee

Kosar Baghbani Parizi

Willy K Wiyatno

Susanne Baumhueter

Janice G Shigeura, Legal Representative

Dennis Lehto

Benjamin G Schroeder

Janusz B Wojtowicz

Douglas Greiner

Jon M Sorenson

Congcong Ma

James C Nurse

Ryoji Inaba

Steven J Boege

Satoshi Takahashi

Zbigniew T Bryning

Tomoyuki Sakai

Jim Nurse

Steven Menchen

Reid Burton Kowallis

Sean Matthew Desmond

Dar Bahatt

Michael S Albin

Richard Morris

Paul O Ramstad

Stephen E Moring

Peter Ma

Dennis E Mead

Ben F Johnson

Dmitry M Sagatelyan

Eric S Nordmann

John H Nickel

Thomas E Lee

Timothy S Orpin

Janice C Woods

Janice Shigeura, Legal Representative

Willy Wiyatno

Dennis Letho

Eric Norman

Sam Lee Woo

Kosar Baghbani Parazi

David M Cox

Zbigniew Bryning

Various embodiments of the teachings relate to a system or method for sample preparation or analysis in biochemical or molecular biology procedures. The sample preparation can involve small volume processed in discrete portions or segments or slugs, herein referred to as discrete volumes. A molecular biology procedure can be nucleic acid analysis. Nucleic acid analysis can be an integrated DNA amplification/DNA sequencing procedure.

Apparatus, system, and method using immiscible-fluid-discrete-volumes

A system may include a first conduit configured to form a first batch of discrete volumes of aqueous fluid separated by spacing liquid disposed between consecutive volumes of aqueous fluid, the spacing liquid being immiscible with the aqueous fluid volumes; a second conduit, fluidically coupled to the first conduit, the second conduit configured to statically hold the first batch of discrete volumes of aqueous fluid; and a third conduit configured to receive the first batch of discrete volumes of aqueous fluid from the second conduit. The third conduit can be configured to transfer the discrete volumes of aqueous fluid of the first batch for downstream processing.

Device and method for making discrete volumes of a first fluid in contact with a second fluid, which are immiscible with each other

The invention relate to systems and methods for sequencing polynucleotides, as well as detecting reactions and binding events involving other biological molecules. The systems and methods may employ chamber-free devices and nanosensors to detect or characterize such reactions in high-throughput. Because the system in many embodiments is reusable, the system can be subject to more sophisticated and improved engineering, as compared to single use devices.

Systems and methods for genetic and biological analysis

A method for generating, within a conduit, discrete volumes of one or more fluids that are immiscible with a second fluid. The discrete volumes can be used for biochemical or molecular biology procedures involving small volumes, for example, microliter-sized volumes, nanoliter-sized volumes, or smaller. The discrete volumes are separated from one another by a liquid that is immiscible with the fluid(s) of the discrete volumes, for example, aqueous immiscible-fluid-discrete volumes separated by an oil.

System and method for fluorescent light excitation and detection from samples to enhance the numerical aperture and/or reduce the cross-talk of the fluorescent light. The system for analyzing samples comprising: a light source that provides a non-coherent excitation light; at least one housing, wherein the housing transports samples and propagates the non-coherent excitation light by total internal reflection; a coupling optical element configured to introduce the non-coherent excitation light into the at least one housing through a wall of the at least one housing; and at least one NA enhancing optical element to collect an emitted fluorescence, wherein the NA enhancing optical element is constructed of a first material and the housing is constructed of a second material, wherein the first material has a greater index of refraction than the second material.

Axial illumination for capillary electrophoresis

Various embodiments relate to systems and/or methods for sample preparation that can be used for biochemical and/or molecular biology procedures involving small volumes, for example, micro volumes or smaller. Methods and systems that can reduce sample size requirements and increase the number of samples on a substrate are provided. Samples can be applied to a plate or other appropriate substrate and can be used for, inter alia, sequencing reactions. In some embodiments, apparatuses, systems, and/or methods for charged analyte collection are provided. Charged analytes in a sample can be electrokinetically collected or extracted from a conduit through a hole formed in a sidewall of the conduit, by application of an electric field that causes the charged analytes to migrate in a direction that is transverse to the conduit.

Device, system, and method for depositing processed immiscible-fluid-discrete-volumes

Growing community of inventors

Hayward, CA, United States of America

Richard T Reel