Dyadic International (usa), Inc.

Evolva, Inc.

Allylix, Inc.

Danisco US Inc.

Arkion Life Sciences, LLC

Dyadic International, Inc.

Dyadic Nederland B.v.

Richard Paul Burlingame

Peter Jan Punt

Mark Aaron Emalfarb

Bryan N Julien

Christine Marie Pynnonen

Arkady Panteleimonovich Sinitsyn

Philip Terry Olson

Jean Christophe Bousson

Martine Parriche

Grace Eunyoung Park

Cornelia Marie Johanna Van Zeijl

Jacob B Visser

Jan Cornelis Verdoes

Jan Wery

Johannes Heinrich Visser

Phillip T Olson

Arie Ben-Bassat

James R Millis

Jean Davin Amick

Cornelia Maria Johanna Van Zeijl

Irina Vladimirovna Solovjeva

Vladimir Mikhaylovich Chernoglazov

Oleg Nicolaevich Ocounev

Mark Aaron Emalfrab

Alan Berry

Cornelia Maria Van Zeijl

The present invention provides a new fungal production system comprising a fungal host strain ofwherein the endogenous cellulase secretion is less than 20% of the endogenous cellulase secretion ofstrain UV 18-25. Preferably, also the secretion of endogenous protease, endogenous &#x3b2;-glucanase and endogenous cellobiohydrolase is less than 20% of the secretion ofstrain UV 18-25. Furthermore, fungal host strains are provided wherein several genes have been disrupted. According to another aspect of the invention a method for homologous and/or heterologous production of a pure protein with a purity of higher than 75%, comprising expressing a gene encoding said protein in the strains according to the invention have been described. Furthermore, a method for production of artificial protein mixes comprising expressing a gene encoding each of said proteins in a strain according to the invention have been disclosed. Finally a method for simplified screening of strains functionally expressing a desired enzyme by application of said strains have been provided.

Fungal production system

Provided are labdenediol diphosphate synthase polypeptides, sclareol synthase polypeptides, nucleic acid molecules encoding the labdenediol diphosphate synthase polypeptides and sclareol synthase polypeptides, and methods of using the labdenediol diphosphate synthase polypeptides, sclareol synthase polypeptides. Also provided are methods for producing labdenediol diphosphate, sclareol and (&#x2212;)-ambroxide.

Sclareol and labdenediol diphosphate synthase polypeptides, encoding nucleic acid molecules and uses thereof

Provided are modified valencene synthase polypeptides and methods of using the modified valencene synthase polypeptides. Also provided are methods for producing modified terpene synthases.

Modified valencene synthase polypeptides, encoding nucleic acid molecules and uses thereof

A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus, more particularly ofand mutants or derivatives thereof. It also covers transformants containingcoding sequences, as well expression-regulating sequences ofgenes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.

Transformation system in the field of filamentous fungal hosts

The present invention is directed to variant squalene synthase enzymes, includingsqualene synthase enzymes, and to nucleic acid molecules encoding these variant enzymes. These variant enzymes produce squalene at a lower rate than the wild-type enzyme, allowing more farnesyl pyrophosphate to be utilized for production of isoprenoid compounds, while still producing sufficient squalene to allow thecells to grow without the requirement for supplementation by sterols such as ergosterol. These variant enzymes, therefore, are highly suitable for the efficient production of isoprenoids.

Method for production of isoprenoid compounds

Isoprenoid compounds

A computer-based method and apparatus for the analysis specification and support of work processes. The system is designed to support multiple interdependent decisions, at least some of which require collaboration among multiple participants (). Work processes are modeled using an application framework () used to develop abstract, decision () process models. The decision () process models are used as a pattern to instantiate concrete process models that incorporate the work defined by the abstract process. The process model is then used to instantiate project models that incorporate the required work from the process. The project models are used to direct and guide the behavior of the participants () in the work process.

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A novel transformation system in the field of filamentous fungal hosts for expressing and secreting heterologous proteins or polypeptides is described. The invention also covers a process for producing large amounts of polypeptide or protein in an economical manner. The system comprises a transformed or transfected fungal strain of the genus Chrysosporium, more particularly of and mutants or derivatives thereof. It also covers transformants containing Chrysosporium coding sequences, as well expression-regulating sequences of Chrysosporium genes. Also provided are novel fungal enzymes and their encoding sequences and expression-regulating sequences.

The present invention relates to a method and materials for producing glucosamine by fermentation of a genetically modified microorganism. Included in the present invention are genetically modified microorganisms useful in the present method for producing glucsamine, as well as recombinant nucleic acid molecules and the proteins produces by such recombinant nucleic acid molecules.

Process and materials for production of glucosamine

Growing community of inventors

Nicholasville, KY, United States of America

Richard Paul Burlingame