Harvard College

The General Hospital Corporation

Tufts University

Richard Novak

Donald Elliot Ingber

Daniel Levner

Norman Wen

Antonio Varone

Justin Nguyen

Lori McPartlin

Youngjae Choe

Lian Leng

David Benson Chou

Arianna Rech

Liliana S Teixeira Moreira Leijten

Oren Levy

Yuka Milton

Viktoras Frismantas

Christopher David Hinojosa

Dennis L Kasper

Geraldine A Hamilton

Josiah Daniel Sliz

Jose Fernandez-Alcon

Remi Villenave

Guy Thompson

Jefferson Puerta

Georg Bauer

Alfred Paris

Karel Domansky

Ulri Nicole Lee

Kambez Hajipouran Benam

Thomas Umundum

Michael Levin

David James Coon

Daniel E Shea

Remco Van Erp

Bret A Nestor

Elizabeth L Calamari

Francesca S Gazzaniga

Michael L Cronce

Sasan Jalili-Firoozinezhad

Diogo M Camacho

Carlos Francisco Ng Pitti

Cicely Fadel

Amir Bein

David Conegliano

Rachelle Prantil-Baun

Liliana Teixeira

Olivier Yves Frederic Henry

Miles Ingram

Tiama Hamkins-Indik

Justin K Nguyen

James Coon

A device for simulating a function of a tissue includes a first structure, a second structure, and a membrane. The first structure defines a first chamber. The first chamber includes a matrix disposed therein and an opened region. The second structure defines a second chamber. The membrane is located at an interface region between the first chamber and the second chamber. The membrane includes a first side facing toward the first chamber and a second side facing toward the second chamber. The membrane separates the first chamber from the second chamber.

Open-top microfluidic devices and methods for simulating a function of a tissue

The present disclosure relates to a microfluidic devices and methods for culturing bone marrow cells. Aspects include methods of preparing microfluidic devices and culturing bone marrow cells with the microfluidic devices. In some aspects, a method includes providing a microfluidic device having an upper chamber, a lower chamber, and a porous membrane separating the upper chamber from the lower chamber. The method further includes seeding walls of the lower chamber and a bottom surface of the membrane with endothelial cells. The method further includes providing a matrix within the upper chamber. The matrix includes fibrin gel and bone marrow cells. The method further includes filling or perfusing the upper chamber with a media.

Bone marrow microfluidic devices and methods for preparing and using the same

An organomimetic device includes a microfluidic device that can be used to culture cells in its microfluidic channels. The organomimetic device can be part of dynamic system that can apply mechanical forces to the cells by modulating the microfluidic device and the flow of fluid through the microfluidic channels. The membrane in the organomimetic device can be modulated mechanically via pneumatic means and/or mechanical means. The organomimetic device can be manufactured by the fabrication of individual components separately, for example, as individual layers that can be subsequently laminated together.

Organomimetic devices and methods of use and manufacturing thereof

A microfluidic device is directed to sustaining a complex microbial community in direct and indirect contact with living human intestinal cells in vitro. The device includes a first microchannel having cultured cells of a human intestinal epithelium and microbiota, the first microchannel further having a first level of oxygen. The device further includes a second microchannel having cultured cells of a vascular endothelium, the second microchannel further having a second level of oxygen. The device also includes a membrane located at an interface region between the first microchannel and the second microchannel, the membrane being composed of an oxygen-permeable material or further having pores via which oxygen flows between the first microchannel and the second microchannel to form a physiologically-relevant oxygen gradient.

Complex human gut microbiome cultured in an anaerobic human gut-on-a-chip

A method for micro-molding a polymeric membrane and including pouring a predetermined volume of curable polymer unto a micro-fabricated mold having a post array with pillars, and overlaying the polymer with a support substrate. A spacer, such as a rubber spacer, is placed in contact with the support substrate and a force is applied to an exposed side of the spacer to compress the support substrate and the polymer together. While applying the force, the polymer is cured on the mold for a predetermined time period and at a predetermined temperature to form a polymeric membrane having a pore array with a plurality of pores corresponding to the plurality of pillars of the post array. The polymeric membrane is removed from the support substrate.

Method for micromolding a polymeric membrane having a pore array

A fluidic device includes a fluidic layer, a capture material, and an electronics layer, the fluidic layer includes a main channel and a pair of sample channels fluidly coupled to the main channel. The pair of sample channels is configured to receive and introduce a sample material into the device. The sample material includes an analyte. The capture material is positioned in a portion of the main channel that is spaced from the pair of sample channels. The capture material has a three-dimensional matrix of receptors therein configured to bond with the analyte. The capture material has a length that is associated with a dynamic range of the fluidic device and a cross-sectional area that is associated with a sensitivity of the fluidic device. The electronics layer includes electrodes configured to measure an electrical resistance through a portion of the capture material.

Universal approach for decoupling sensitivity and dynamic range of a sensor

Described herein are microfluidic devices and systems for high density cell culture and/or high throughput cell assays. Methods of using the same are also provided herein. In some embodiments, the microfluidic devices and systems described herein provide rapid and automated trapping of single embryos in ordered arrays.

Microfluidic devices and systems for cell culture and/or assay

A microfluidic device for simulating a function or response of a tissue is disclosed. The device includes an inlet for receiving a fluid in the device, and an outlet for removing the fluid from the device. The device further includes a fluid channel in fluid communication with the inlet and the outlet for flowing the fluid through the device. The fluid channel defines a chamber well that receives cells associated with the tissue. The device also includes an interface structure between the fluid channel and the chamber well for permitting migration of at least one of cells, particulates, chemicals, molecules, liquids, or gases between the fluid within the fluid channel and the chamber well.

Radial microfluidic devices and methods of use

Growing community of inventors

Boston, MA, United States of America

Richard Novak