Labcyte Inc.

Picoliter Inc.

Xerox Corporation

Richard N Ellson

Mitchell Wayne Mutz

Richard G Stearns

Babur B Hadimioglu

James K Foote

Joseph D Olechno

David Allen Mantell

Richard Caprioli

David Soong-Hua Lee

Robert Arthur Sprague

Joy Roy

Robert G Kuimelis

James Bernard Kruger

Michael F Miller

John M Lennon

Marsha N Blauwkamp

Jennifer M Hardee

Arlen Mark Bramwell

David Kalman Biegelsen

Jeffrey J Folkins

Scott Alan Elrod

Steven A Buhler

Stephen David White

Shehrzad Ahmed Qureshi

Jay Daulton

Robert P Kowalski

Michael Y Young

Thanh D Truong

Lars Majlof

Ian Sinclair

Theodore J Williams

Jonathan Wingfield

Richard M Caprioli

Sample container for holding and transferring a liquid sample and method thereof. The sample container includes an inlet configured to allow a liquid sample to enter a sample container, and an outlet configured to allow one or more droplets of the liquid sample to exit the sample container by one or more acoustic ejections respectively. The inlet and the outlet are in different locations.

Sample containers adapted for acoustic ejections and sample preservation and methods thereof

Systems and methods described herein employ focused acoustic energy applied to a reservoir containing a fluid to eject a fluid sample from the fluid sample reservoir, e.g. to an inlet of an analytical device. In many embodiments, the ejected fluid sample traverses an air gap separating the inlet of the analytical device from an upper surface of the fluid in the fluid sample reservoir. In many embodiments, the ejected fluid sample comprises one or more droplets ejected from the fluid sample reservoir, which can contain particles suspended in the fluid sample.

Acoustic concentration, transfer and analysis of samples containing particles

A fluid impermeable transducer includes an assembly of a transducer head and a casing, and an actuator disposed in the casing rearward of the back of the transducer head and operable to transmit acoustic energy through the transducer head. The transducer head and casing define a working portion of the transducer that is fluid impermeable.

Fluid impermeable ultrasonic transducer

Provided herein is generally tubular container, preferably including a plurality of reservoirs defined therein. The container can be adapted for acoustic ejection of a fluid disposed within at least one of the reservoirs of the plurality of reservoirs. Alternatively, the container can be adapted for extraction of a fluid disposed within at least one of the reservoirs of the plurality of reservoirs using a non-acoustic liquid handling method.

Roughly cylindrical sample containers having multiple reservoirs therein and being adapted for acoustic ejections

A method and system are provided for extracting a target analyte from a sample using acoustic ejection technology. The method involves applying focused acoustic energy to a fluid reservoir housing a fluid composition that contains a target analyte and comprises an upper region and a lower region, where the concentration of the target analyte in the upper region differs from that in the lower region. The focused acoustic energy is applied in a manner that is effective to result in the ejection of a fluid droplet from the fluid composition into a droplet receiver, wherein the concentration of the analyte in the droplet corresponds to either the concentration of the analyte in the upper region or the concentration of the analyte in the lower region, and wherein the concentration of the analyte is substantially uniform throughout the droplet. The fluid composition may comprise an ionic liquid, used in the extraction of ionic target analytes. Related methods and an acoustic extraction system are also provided.

System and method for extracting a target moiety from a sample using acoustic droplet ejection

A method is provided for achieving transfection of host cells using sonoporation. An acoustic radiation generator is positioned in acoustic coupling relationship with respect to a reservoir containing host cells to be transfected, exogenous material to be incorporated into the host cells, and a cell-compatible fluid medium. The acoustic radiation generator is activated to generate acoustic radiation and direct the acoustic radiation into the reservoir in a manner effective to enable transfection of the host cells with the exogenous material.

High-efficiency transfection of biological cells using sonoporation

A method and system are provided for extracting a target analyte from a sample using acoustic ejection technology. The method involves applying focused acoustic energy to a fluid reservoir housing a fluid composition that contains a target analyte and comprises an upper region and a lower region, where the concentration of the target analyte in the upper region differs from that in the lower region. The focused acoustic energy is applied in a manner that is effective to result in the ejection of a fluid droplet from from the fluid composition into a droplet receiver, wherein the concentration of the analyte in the droplet corresponds to either the concentration of the analyte in the upper region or the concentration of the analyte in the lower region, and wherein the concentration of the analyte is substantially uniform throughout the droplet. The fluid composition may comprise an ionic liquid, used in the extraction of ionic target analytes. Related methods and an acoustic extraction system are also provided.

Devices and methods are provided for ejecting a droplet from a reservoir using focused acoustic radiation having a plurality of nonsimultaneous and discrete frequency ranges. Such frequency ranges may be used to control droplet volume and/or velocity. Optionally, satellite fluid ejection from the reservoir is suppressed.

Droplet ejection using focused acoustic radiation having a plurality of frequency ranges

A container may include a tubular sidewall defining interior and exterior surfaces of the container, and including first and second regions disposed relative to one another along a major axis of the tubular sidewall. The container further may include an identification mark embedded within the tubular sidewall at a plurality of sectors about the tubular sidewall within the first region. Each sector may have a width, and the identification mark is machine readable by a reader viewing any arbitrary one or more of the sectors. An exemplary method for preparing such a container is also provided.

Sample containers having identification marks embedded therein and being adapted for acoustic ejections

A method and system are provided for detecting the concentration of an analyte in a fluid sample. The method and system involve analysis of a volatilized, ionized fluid sample using a mass spectrometer or other ionic analyte detection device that provides a signal proportional in intensity to the quantity of ionized analyte detected. The improvement involves replacement of a necessary non-analyte component in the fluid sample with a substitute component that serves the same purpose as the original component but is either more volatile than the original component and/or the analyte or undergoes a reaction to provide lower molecular weight reaction products, and results in an increased intensity in signal and signal-to-noise ratio. Acoustic fluid ejection is a preferred method of generating nanoliter-sized droplets of fluid sample that are then volatilized, ionized, and analyzed. Also provided are zwitterionic compounds suitable as the substitute components that when ionized and heated decompose to provide carbonic dioxide, a nitrogenous species such as ammonia, an amine, or nitrogen gas, and a volatile aromatic compound.

Method and system for determining the concentration of an analyte in a fluid sample

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Palo Alto, CA, United States of America

Richard N Ellson