Illinois Institute of Technology

Northfield Laboratories, Inc.

USA As Represented by Secretary of the Navy

Richard L Beissinger

Arthur L Rosen

Darsh T Wasan

Lakshman R Sehgal

Martha C Farmer

Lahksman R Sehgal

Shuming Zheng

A process is provided wherein hemoglobin, a fragile material, is formulated into high hemoglobin content water-in-oil-in-water multiple emulsion while maintaining high yields and high oxygen exchange activity. A multiple emulsion of aqueous oxygen carrying material in oil in outer aqueous phase is suitable for provision of oxygen for oxygen transfer processes. A hemoglobin multiple emulsion in physiologically compatible oil in an outer aqueous saline solution is provided in sufficiently small droplet size to provide oxygen flow through blood vessels to desired body tissues or organs thereby providing a blood substitute.

Oxygen carrying multiple emulsions

Process for preparation of hemoglobin multiple emulsions

A multiple emulsion of aqueous oxygen carrying material in oil in outer aqueous phase is suitable for provision of oxygen for oxygen transfer processes. A hemoglobin multiple emulsion in physiologically compatible oil in an outer aqueous saline solution is provided in sufficiently small droplet size to provide oxygen flow through blood vessels to desired body tissues or organs thereby providing a blood substitute. A process is provided wherein hemoglobin, a fragile material, is formulated into high hemoglobin content water-in-oil-in-water multiple emulsions while maintaining high yields and high oxygen exchange activity.

Hemoglobin multiple emulsion

Sterile liposome-encapsulated hemoglobin having an oxygen carrying capacity of 20 vol/%, a half life of 15-20 hours as measured in mice, with the liposome made from chloroform, HSPC, cholesterol, negatively charged DMPG and alpha-tocopherol.

Blood substitute comprising liposome-encapsulated hemoglobin

A method for making sterile liposome-encapsulated hemoglobin having an oxn carrying capacity of 20 vol/%, a half life of 15-20 hours as measured in mice, with the liposome made from chloroform, HSPC, cholesterol, negatively charged DMPG and alpha-tocopherol.

Growing community of inventors

Oak Park, IL, United States of America

Richard L Beissinger