Monsanto Technology LLC

Other

Richard J Lawrence

Ervin D Nagy

Linda Rymarquis

Jonathan C Lamb

Andrei Y Kouranov

Brent Brower-Toland

Rosemarie Kuehn

Veena Veena

Xudong Ye

Paul S Chomet

Elysia K Krieger

Fenggao Dong

Edward James Cargill

Huachun Wang Larue

Jianping Xu

Charles L Armstrong

Thomas S Ream

Zarir E Vaghchhipawala

Isabel Clare Lawrence

The disclosure provides novel corn, tomato, and soybean U6, U3, U2, U5, and 7SL snRNA promoters which are useful for CRISPR/Cas-mediated targeted gene modifications in plants. The disclosure also provides methods for use for U6, U3, U2, U5, and 7SL promoters in driving expression of sgRNA polynucleotides which function in a CRISPR/Cas system of targeted gene modification in plants. The disclosure also provides methods of genome modification by insertion of blunt-end DNA fragments at a site of genomic cleavage.

Compositions and methods for site directed genomic modification

The present disclosure is in the field of plant transformation. The disclosure provides methods for increasing the rate of site-directed integration of a sequence of interest in plants. The disclosure also provides methods for increasing the efficiency of Rhizobiales-mediated plant transformation.

Methods and compositions for use in genome modification in plants

Methods and compositions for improved plant breeding using gene editing and haploid induction are provided.

Methods and compositions for genome editing via haploid induction

The current invention provides methods for creating a block of genetically linked transgenic traits or a megalocus that can be transmitted as a single genetic unit. The present invention further provides methods for trait introgression to other plants, varieties or species using the megaloci of the invention. Also provided are plants, seeds, and plant parts comprising the megaloci.

Creation and transmission of megaloci

The invention relates to biotechnology and provides novel methods for sequence-specific or sequence-directed transcription activator-like effector recombinase-mediated integration of DNA sequences of interest into host genomes. The invention also provides methods of use for novel plant transformation vectors and expression cassettes, which include novel combinations of chimeric recombinases with plant expression and transformation elements. Methods for gene-targeting, DNA sequence removal, genome modification, and molecular breeding are also provided.

Methods for targeted transgene-integration using custom site-specific DNA recombinases

Eyeglasses visor and case

Golf club holder

Nestable software license authentication keys connect end-to-end on a circuit board to form an authentication key assembly for internal computer installation or for use as an external assembly connecting to a host system. The circuit board includes at least one plastic housing for one or more authentication keys which define a male connector at one end and a female connector at the opposite end, which connect to a female phone jack(s) electrically connected to the circuit board and attached to the end of a compression spring mounted within each housing. The spring(s) compresses as additional keys are connected end-to-end on the board and the spring biases the keys against the end of the circuit board. The authentication key assemblies may utilize a variety of circuit boards with a variety of connectors, and the external assembly is encased in the plastic housing for use with portable and Apple-type computers.

Hardware mechanism for computer software security

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Kirkwood, MO, United States of America

Richard J Lawrence