Abbott Laboratories Corporation

Richard Haack

Qiaoqiao Ruan

Sergey Y Tetin

Mark R Pope

M Felicia Bogdan

Xiaoxing Qiu

Zhen Lin

Kerry M Swift

Brenda Calfin

Patrick J Macdonald

Mark A Hayden

Andrew G Fischer

Anthony Scott Muerhoff

Jeffrey B Huff

Thomas P Leary

John C Prostko

Sophie Laurenson

Shelley R Holets-McCormack

Pathik H Soni

Lyle Yarnell

Peter J Karabatsos

Edna Prieto-Ballengee

Dustin House

Lei Qiao

M Shawn Murphy

Stefan Hershberger

Andrew S Schapals

Andrew T Fischer

Edna M Prieto-Ballengee

Felicia Bogdan

Thomas Leary

The disclosure provides methods of analyzing an analyte of interest in a biological sample using fluorescent agents and macroconjugates which comprise a core containing a cross-linked polymer or protein, tags, specific binding members or fragments thereof, and optionally carrier proteins. Also provided are methods of analyzing two or more analytes of interest in a biological sample in a single assay using microparticles and detection conjugates comprising different fluorophore labels, acquiring transmitted light and fluorescent images of the microparticles, and using a customized image analysis process to analyze the acquired images.

Direct detection of single molecules on microparticles

Methods, devices, and systems for analyte analysis using a nanopore are disclosed. The methods, devices, and systems utilize a first and a second binding member that each specifically bind to an analyte in a biological sample. The method further includes detecting and/or counting a cleavable tag attached to the second binding member and correlating the presence and/or the number of tags to presence and/or concentration of the analyte. Certain aspects of the methods do not involve a tag, rather the second binding member may be directly detected/quantitated. The detecting and/or counting may be performed by translocating the tag/second binding member through a nanopore. Devices and systems that are programmed to carry out the disclosed methods are also provided.

Devices and methods for sample analysis

Growing community of inventors

Skokie, IL, United States of America

Richard Haack