University of California

Axys Pharmaceuticals, Inc.

Richard H Lathrop

G Wesley Hatfield

David Chapman

Tomas Lozano-Perez

Ajay N Jain

Roger Critchlow

Rommie E Amaro

Peter Kaiser

Thomas Glen Dietterich

Roberta Baronio

Seyedey-Faezeh Salehi-Amin

Ozlem Demir

Christopher D Wassman

A method of enhancing p53 activity of a p53 mutant polypeptide is provided. The method includes interacting a compound to an open L1/S3 binding site of the p53 mutant polypeptide, where the p53 activity of the p53 mutant polypeptide is enhanced in the presence of the compound. Compounds were identified from databases of compounds for virtual drug screening. Methods of screening for compounds that enhance p53 activity by binding to the open L1/S3 binding site, and methods of treatment using the identified compounds, are also provided.

Small molecules to enhance P53 activity

Disclosed herein is a method for synthesizing a desired nucleic acid sequence. The method comprises dividing the desired sequence into a plurality of partially overlapping segments; optimizing the melting temperatures of the overlapping regions of each segment to disfavor hybridization to the overlapping segments which are non-adjacent in the desired sequence; allowing the overlapping regions of single stranded segments which are adjacent to one another in the desired sequence to hybridize to one another under conditions which disfavor hybridization of non-adjacent segments; and filling in, ligating, or repairing the gaps between the overlapping regions, thereby forming a double-stranded DNA with the desired sequence. Also disclosed is a method for preventing errors in the synthesis of the nucleic acid sequence.

Method for producing a synthetic gene or other DNA sequence

Machine-learning approach to modeling biological activity for molecular

Growing community of inventors

Irvine, CA, United States of America

Richard H Lathrop