Leland Stanford Junior University

Affymetrix, Inc.

Eos Biotechnology, Inc.

Richard Glynne

David H Mack

Ghassan Ghandour

Chris Goodnow

The invention provides for the identification of all genes, whether known or novel, which are differentially expressed within and among B cells, making possible the characterization of their temporal regulation and function in the B cell response and/or in B cell mediated disorders. Expression profiles, nucleic acids and proteins are provided for differing states of B cells, including resting, naive, activated, tolerant and immunosuppressed B cells. The present invention makes possible the identification and characterization of targets useful in prognosis, diagnosis, monitoring, rational drug design, and/or therapeutic intervention of immune system disorders.

Methods of screening for B cell activity modulators

Computer programs and computer-implemented methods are disclosed for extracting, analyzing and comparing data from biomolecular binding experiments. The invention receives a set of values corresponding to the interaction of one or more target samples with probes on one or more arrays of biomolecular probes, and uses the values to generate one or more probability values indicating the probability that a molecular species complementary to the probe set is not present in the target samples, or is present at levels greater than or less than one or more reference levels. The probability values are generated by comparing the values for individual probes in a probe set with values for a calibration set, which may include a null set, spiked probe sets, or housekeeping probe sets. Normalized experimental values are generated for sets of probes on the arrays using statistical methods. Data is output in the form of data files including normalized experimental values and probability values for probe sets in the arrays.

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Palo Alto, CA, United States of America

Richard Glynne