Bayer Cropscience Ag

Hoechst Schering Agrevo Gmbh

Cooperatieve Verkoop-en Productievereniging Van Aardeppelmeel En Derivaten Abebe, B.a.

Richard G F Visser

Claus Frohberg

Jean-Paul Vincken

Geraldine Kok-Jacon

Luc C J M Suurs

Jens Kossmann

Uwe Sonnewald

Lothar Willmitzer

Evert Jacobsen

Evert Jacobson

Christiann J J Raemakers

Johanna E M Bergervoet Van Deelan

Bernd M&uuml;ller-R&ouml;ber

The present invention relates to plant cells and plants, which are genetically modified, wherein the genetic modification leads to the expression in plastids of such plant cells and plants of an enzyme having the activity of a mutansucrase. Furthermore, the present invention relates to means and methods for the manufacture of such plant cells and plants. Plant cells and plants of this type synthesise a modified starch. The present invention therefore also relates to the starch synthesised by the plant cells and plants according to the invention as well as to methods for the manufacture of the starch and to the manufacture of starch derivatives of this modified starch.

Transformed plant expressing a mutansucrase and synthesizing a modified starch

The present invention relates to plant cells and plants, which are genetically modified, wherein the genetic modification leads to the expression in plastids of such plant cells and plants of an enzyme having the activity of a dextransucrase. Furthermore, the present invention relates to means and methods for the manufacture of such plant cells and plants. Plant cells and plants of this type synthesis a modified starch. The present invention therefore also relates to the starch synthesized by the plant cells and plants according to the invention as well as to methods for the manufacture of the starch and to the manufacture of starch derivatives of this modified starch.

Transformed plant expressing a dextransucrase and synthesizing a modified starch

The invention relates to a method for producing protoplasts of cassava or closely related species, which protoplasts are capable of regeneration into plants. The method comprises producing friable embryogenic callus from explants of cassava or closely related species and isolating protoplasts from said friable embryogenic callus. The invention also concerns protoplasts obtainable by said method. The invention further relates to a method for transforming such a protoplast of cassava or closely related species, and transformed protoplasts obtainable thereby. In addition, the invention concerns a method for regenerating plants from these protoplasts and a cassava plant or closely related species obtainable thereby. Starch isolated from tubers of such cassava plants have an increased amylopectin content. The starch and a method for isolating it from said plants also forms part of the invention.

Methods for producing and transforming cassava protoplasts

Plasmids are described having DNA sequences that after insertion into the genome of the plants cause changes in the carbohydrate concentration and the carbohydrate composition in regenerated plants. These changes can be obtained from a sequence of a branching enzyme that is located on these plasmids. This branching enzyme alters the amylose/amylopectin ratio in starch of the plants, especially in commercially used plants.

Plasmids containing DNA-sequences that cause changes in the carbohydrate concentration and carbohydrate composition in plants, as well as plant cells and plants containing these plasmids

Growing community of inventors

Bennekom, Netherlands

Richard G F Visser