Valtion Teknillinen Tutkimuskeskus

Ab Enzymes Gmbh

Ab Enzymes Oy

Richard Fagerström

Maria-Regina Kula

Marja T Paloheimo

Pirkko Suominen

Jari Vehmaanperä

Terhi Puranen

Leena Valtakari

Merja Penttilä

Pentti Ojapalo

Jarno Kallio

Tiina Nakari-Setälä

Markus Linder

Klaus Selber

Folke Tjerneld

Raija Lantto

Tarja Lahtinen

Kristiina Kruus

Arja Mäntylä

Sanna Leskinen

The present invention is related to an improved production of bacterial proteins in filamentous fungus, e.g. inand. The improvement is achieved by constructing expression vectors, which comprise the bacterial protein encoding DNA sequences fused in frame with a DNA sequence encoding a filamentous fungus secretable protein or one or more functional domains of said protein. Filamentous fungus hosts transformed with such expression vectors secrete the desired proteins or enzymes, especially xylanases or cellulases originating from bacteria or more preferably from actinornycetes into the culture medium of the host. The desired proteins or enzymes can be used directly from the culture medium after separation of host cells or recovered and treated using down-stream processes, which are appropriate for the respective application. Xylanases or cellulases from actinomycetes produced using the above expression vectors are most suitable for treating plant derived materials e.g. in pulp and paper industries.

Production and secretion of proteins of bacterial origin in filamentous fungi

The present invention relates to isolation and purification of protein in aqueous two-phase systems (ATPS). Specifically, the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.

Process for partitioning of proteins

The present invention relates to isolation and purification of proteins in aqueous two-phase systems (ATPS). Specifically the invention provides processes for partitioning of proteins of interest in ATPS by fusing said proteins to targeting proteins which have the ability of carrying said protein into one of the phases.

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Espoo, Finland

Richard Fagerström