Plant Bioscience Limited

Seminis Vegetable Seeds, Inc.

Richard F Mithen

Bart Willem Brugmans

Maria Traka

Franciscus Van Den Bosch

Gerard N Koorevaar

Bart W Brugmans

Kathy Faulkner

Gary Williamson

Franciscus G Van Den Bosch

The invention provides compositions and methods relating to the elevation of glucoraphanin compared to standardvarieties. The invention also relates to the production of hybrid varieties having desired glucosinolate contents. The invention further provides plants, plant parts, and seeds comprising such traits and comprising a Myb28 allele fromthat is not genetically linked to an ELONG allele from

plants with improved nutritional value

The present invention relates to a method for determining the genotype of a Cruciferous vegetable plant for a plant with an increased glucosinolate level, comprising obtaining a sample of nucleic acids from said plant or a portion thereof and detecting in said nucleic acids a polymorphism at the Myb28 locus that is genetically linked to an increased glucosinolate level. The polymorphism may comprises at least one of: a) a single nucleotide polymorphism (SNP) at a position corresponding to nucleotide 83, 136, 226, 563, 610, 830, 995, 1116, 1513, 1577, 1606, 1620, 1825, 1863, 1877 or 2026 of SEQ ID NO: 1, or b) a polymorphism in the number of nucleotides present between nucleotides 323 and 332, between nucleotides 521 and 524, between nucleotides 783 and 786, between nucleotides and 909 and 914, between nucleotides 1365 and 1369, between 1811 and 1821, or between nucleotides 2046 and 2056 of SEQ ID NO: 1, or c) a polymorphism in the number of nucleotides present between nucleotides 836 and 837, between nucleotides 867 and 868, or between nucleotides 943 and 944 of SEQ ID NO: 1.

Genetic markers for Myb28

A composition comprising glucoraphanin for use in: reducing fasting serum glucose levels in a subject administered with said composition when compared to a subject not administered said composition; and/or preventing diabetes mellitus and/or a condition associated therewith, treating diabetes mellitus and/or a condition associated therewith, or a combination thereof in a subject; wherein the composition is intermittently administered to the subject between about 1 to about 5 times per week such that said subject is administered with a weekly dose of between about 300 to about 2500 &#x3bc;moles of glucoraphanin, as well as uses of a composition prepared from a high glucosinolate broccoli and/or methods of: reducing fasting serum glucose levels in a subject having elevated fasting serum glucose levels; preventing diabetes mellitus and/or a condition associated therewith in a subject; treating a subject having diabetes mellitus and/or a condition associated therewith; or combinations thereof.

Glucoraphnin for use in the treatment and/or prevention of diabetes mellitus

The present invention relates to a composition comprising a high glucosinolate Cruciferous vegetable (such as a high glucosinolate broccoli) having a high level of glucosinolate and/or at least one derivative thereof for use in the treatment or prevention of a cardiovascular disease or for use in promoting heart and/or cardiovascular health.

Method for treating a cardiovascular disease, hypercholesterolemia or hypertension or improving serum cholesterol levels by administering broccoli with a high level of glusosinolates

GSL-ELONGASE gene ofand other plants, especiallyand. The nature and level of glucosinolates in plants can be modified by transgenic expression of the genes or control of the level of expression.

Methods and means for modification of glucosinolates in plants

The invention relates to a method for producing with elevated anticarcinogenic glucosinolate derivatives. The elevated levels are obtained by crossing wild species with breeding lines, and subsequently selecting hybrids with levels of 4 -methylsulfinylbutyl and/or 3-methylsulfinylpropyl glucosinolates elevated above that initially found in breeding lines. The invention also relates to edible Brassica plants, such as broccoli plants, with elevated levels of 4-methylaulfinylbutyl glucosinolate and/or 3 -methylsulfinylpropyl glucosinolates, and to seeds of such plants.

Growing community of inventors

Norwich, United Kingdom

Richard F Mithen