Mycogen Plant Science, Inc.

Pioneer Hi-Bred International, Inc.

Richard F Barker

John D Kemp

Michael J Adang

Timothy C Hall

L Sue Loesch-Fries

Nancy P Jarvis

The sequence of the T-DNA of the octopine-type Ti plasmid found in Agrobacterium tumefaciens ATCC 15955 is disclosed. Fourteen open reading frames bounded by eukaryotic promoters, ribosome binding sites, and polyadenylation sites were found. The use of promoters and polyadenylation sites from pTi15955 to control expression of foreign structural genes is taught, using as examples the structural genes for the Phaseolus vulgaris storage protein phaseolin, P. vulgaris lectin, thaumatin, and Bacillus thuringiensis crystal protein. Vectors useful for manipulation of sequences of the structural genes and T-DNA are also provided.

Polypeptides encoded by octopine T-DNA open reading frames

Octopine T-DNA structural genes

Octopine T-DNA promoters

A method is disclosed for detecting RNA viral infections in plants comprising the isolation of an RNA molecule, e.g., from alfalfa mosaic virus (AMV), production of full-length cDNA transcripts, cloning into plasmids and propgation of the recombinant plasmid. This recombinant DNA plasmid is utilized diagostically to test a plant for infection by a virus, e.g., AMV.

Plant virus cDNA

Growing community of inventors

Montigny-le-Bretonneux, France

Richard F Barker