Abbott Laboratories Corporation

Richard E Thompson

Julian D Gordon

Andreas A Kapsalis

Methods and kits are provided for the quantitative determination of the presence of an enzyme analyte in a sample by determination of the rate of analyte catalyzed reaction on a chromatographic medium. The methods may be used to determine both steady state and non-steady state enzyme reaction kinetics and provide a physical record reflecting the same. In a preferred embodiment, an analyte enzyme in a sample to be analyzed is immobilized at a reaction site on a chromatographic medium, the chromatographic medium is contacted with a solution containing a substrate, the solution is transported on the chromatographic medium to the reaction site where the analyte enzyme catalyzes the reaction of the substrate to produce a detectable reaction product at a rate related to the amount of enzyme present, and the solution and reaction product are transported from the reaction site to a detection region formed by a length of the chromatographic medium downstream from the reaction site. Transport continues until the solution reaches the end of the chromatographic medium or until the quantity of solution is exhausted.

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Libertyville, IL, United States of America

Richard E Thompson