Roche Sequencing Solutions, Inc.

Kapa Biosystems, Inc.

Richard Dannebaum

Brian Christopher Godwin

Jan Berka

Sedide Ozturk

Joseph Platzer

Martin Ranik

Khai Luong

Seoyoung Kim

Carolina Dallett

Ashley Hayes

Florian Rubelt

Severine Margeridon

Jainee Lewis

Daleen Badenhorst

Monica Herrera

Michael S Berry

Etienne Slabbert

Dilduz Telman

The invention is a novel method of sequencing nucleic acids involving making and sequencing a library of single stranded circular target nucleic acids.

Generation of single-stranded circular DNA templates for single molecule sequencing

Methods and compositions are described herein for primer extension target enrichment of immune receptor (BCR or TCR) sequences.

Immune profiling by primer extension target enrichment

The invention is a novel method of making and using a library such as a sequencing library of single stranded circular nucleic acid templates via splint ligation. In particular, disclosed are methods of making circular target nucleic acid molecules and libraries of such molecules for downstream analysis such as nucleic acid sequencing. The method comprises the steps of adding universal sequences to nucleic acid molecules, rendering single-stranded these nucleic acid molecules with universal sequences on their ends by contacting with a probe complementary to at least a portion of the universal sequences, and allowing the hybridized probe to enable circularization and formation of single-stranded circular (sscDNA) molecules.

Hybridization-extension-ligation strategy for generating circular single-stranded DNA libraries

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Pleasant Hill, CA, United States of America

Richard Dannebaum