Other

Applied Biosystems, LLC

Life Technologies Corporation

Ambion, Inc.

Richard Conrad

Emily Zeringer

Alexander Vlassov

Mu Li

Brittan L Pasloske

Matthew M Winkler

Gary J Latham

Robert Setterquist

Jon Kemppainen

Xingwang Fang

Marie Gonzalez

The present invention concerns the use of methods and compositions for the isolation of small RNA molecules (100 nucleotides or fewer), such as microRNA and siRNA molecules. Such molecules are routinely lost in commonly used isolation procedures and therefore the present invention allows for a much higher level of enrichment or isolation of these small RNA molecules.

Methods and compositions for isolating small RNA

Methods of directly isolating nucleic acid from an embedded biological sample are provided. An emulsified digest is generated in the presence of a thermostable protease, and an additive selected from an alkylene glycol, a poly(alkylene glycol), or a block copolymer having an average Mof 76 to 2900, or a salt or derivative or combination thereof. Nucleic acid is isolated directly from the emulsified digest. The methods eliminate the use of organic solvents such as xylene in a deparaffinization step prior to isolating nucleic acids from paraffin-embedded samples, for example.

Alkylene glycols and polymers and copolymers thereof for direct isolation of nucleic acid from embedded samples

Methods and compositions for isolating small RNA molecules

Disclosed are methods, compositions and kits for the isolation of exosomes from biological fluids and tissues. Volume-excluding polymers are used to precipitate exosomes from biological samples thereby allowing exosome isolation by low-speed (benchtop) centrifugation or filtration. Further fractionation of exosomes after precipitation is also described.

Methods and compositions for exosome isolation

The invention relates to methods of separating polynucleotides, such as DNA, RNA and PNA, from solutions containing polynucleotides by reversibly binding the polynucleotides to a solid surface, such as a magnetic microparticle.

Modified surfaces as solid supports for nucleic acid purification

The present invention concerns methods and compositions to prepare biological samples to preserve the macromolecules in the samples. Embodiments of the invention concern the use of a soak solution that contains one or more water-miscible solvents. A sample is incubated with the soak solution to the point of saturation at a temperature above the melting temperature of the water-miscible solvent but below 0&#xb0; C. The use of methods and compositions of the invention allow for subsequent preparation or analysis of the samples.

Compositions and methods for preserving RNA in biological samples

The present invention provides methods and compositions, including kits, for the isolation and purification of mRNA, particularly poly(A) RNA. It concerns the use of isostabilizing salts such as TMAC and TEAC to reduce rRNA carryover during the purification process, thus facilitating the isolation of poly(A) RNA.

High efficiency mRNA isolation methods and compositions

A method employing a precharging pulse to adjust and/or to equalize the peak voltages of a discontinuous bipolar pulse waveform used for powering a corona in an ozone generator system. This precharging pulse is generally opposite in polarity to the first unipolar pulse of the bipolar pulse waveform, and precharges the inductances and capacitances of the system to provide priming energy for the first corona pulse and to compensate for skewing of the waveform due to resonances. This method enables the corona to be operated at the optimum voltage for efficient ozone generation, and provides the ability to fine-tune each system to best match high-voltage transformers to corona cells.

Method of powering corona discharge in ozone generators with bipolar

A flat plate corona cell for generating ozone with a generally convergent radial gas flow. The net gas flow direction through the corona gap is from the outer edges of the cell inward towards an exit port in the approximate center of the cell. This eliminates parasitic arcing at the perimeter, greatly increases the life of the perimeter seal, and provides a homogenous gas flow which increases ozone output. The cell design allows both electrodes to be operated at high voltage, therefore reducing the effective kilovolts with respect to ground by half.

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Austin, TX, United States of America

Richard Conrad