Sri International

Richard C Winant

Paul H Johnson

Jerome B Lazar

Ping Sze

The present invention provides a process for the recovery of heterologous proteins from CTAP-III fusion proteins comprising expressing a fusion protein having a first amino acid sequence, a second amino acid sequence, and a selectable site which may be cleaved to provide first and second polypeptide fragments, respectively, wherein the first amino acid fragment is homologous to CTAP-III, and the first and second fragments have different pI values; cleaving the fusion protein to provide the first and second fragments; and separating the first and second fragments by ion exchange chromatography.

Purification of proteins employing CTAP-III fusions

Compounds and compositions comprising amino acid substituted and chemically modified analogs of hirudin are provided, together with methods for their production and use as antithrombotic and anticoagulant agents.

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Palo Alto, CA, United States of America

Richard C Winant