E.i. Dupont De Nemours and Company

E. I. Du Pont Nemours and Co.

Richard C Ebersole

Jianzhong Zhang

Christian Peter Lenges

Mario W Chen

Robert P Foss

Michael D Ward

Edwin R Hendrickson

Frank T Gelormini

Jesse G Forsythe, Jr

Walter R Hertler

Ira B Dicker

Raymond E Jackson

Fritz P Boettcher

John Richard Moran

Mark Scott Payne

Rick Weizhang Ye

James M Odom

Michael P Perry

Randy M McCormick

Scot D Abbott

Linda Jane DeCarolis

Laura Anne Bedzyk

Stephen Varkey

David K Chu

Len J Gawel

Jan Antoni Rafalski

Hui Hsiung

Daniel R Demarco

William R Majarian

John R Moran

Thomas K Ng

Sandra G Fitzpatrick-McElligott

Neel V Neelkantan

John G DeCarolis

Tina Marie Hatfield

David Nash Collier

Daniel DeMarco

Jeffrey Allen Rivenbark

Luis Baez

This invention relates to a method, kit and system for collecting and processing of samples to release and treat DNA and RNA for gene sequence detection. The method described here in provides for rapid and convenient release, and recovery of DNA and RNA from tissues and cellular materials.

Method, kit and system for collecting and processing samples for DNA and RNA detection

Primers have been isolated that are diagnostic for the detection of the white spot syndrome virus (WSSV). The primers are based on a new portion of the WSSV genome and may be used in primer directed amplification or nucleic acid hybridization assay methods.

Sequences diagnostic for shrimp pathogens

Primers have been isolated that are diagnostic for the detection of. The primers are based on a portion of theLuxR gene and may be used in primer directed amplification or nucleic acid hybridization assay methods.

Primers have been isolated that are diagnostic for the detection of the taura syndrome virus (TSV). The primers are based on a new portion of the TSV genome and may be used in primer directed amplification or nucleic acid hybridization assay methods.

Methods and materials for the detection of the foot and mouth disease virus (FMDV). The methods may utilize PCR amplification, with or without an internal positive control, and appropriate primer pairs. The reagents to perform these methods can be supplied as a kit and/or in tablet form.

Sequences diagnostic for foot and mouth disease

Primers have been isolated that are diagnostic for the detection of the infectious hypodermal and hematopoietic necorsis virus (IHHNV). The primers are based on a new portion of the IHHNV genome and may be used in primer directed amplification or nucleic acid hybridization assay methods.

Methods for the direct detection of diagnostic target RNA have been developed, which obviate the need for time consuming RNA purification and isolation procedures.

Method for the direct detection of diagnostic RNA

A unique 16S rRNA profile derived fromhas been identified and isolated. The profile contains several nucleic acid fragments that are linked to dechlorinating activity. These sequences are set forth in SEQ ID NO:1, SEQ ID NO:8, SEQ ID NO:30, and SEQ ID NO:34.

Nucleic acid fragments for the identification of dechlorinating bacteria

A 16S rRNA profile derived from has been identified and isolated. The profile contains several nucleic acid fragments that are linked to dechlorinating activity. These sequences are set forth in SEQ ID NO:1, SEQ ID NO:8, SEQ ID NO:30 and SEQ ID NO:34.

A process is disclosed for the detection of an analyte utilizing a nucleic acid label as a reporter. The analyte is detected by the binding of at least two reporter conjugates, each conjugate comprising a member of a binding pair and a nucleic acid label. The binding of the reporter conjugates to the analyte facilitates the juxtaposition of the nucleic acid labels, forming a single nucleic acid amplicon. The amplicon may then be detected directly, or may be used as a template of the generation of amplification products. Detection of the analyte by this process significantly reduces assay background caused by non-specific reporter conjugate binding.

Method for the detection of an analyte by means of a nucleic acid reporter

A method for detecting the presence of a nucleic acid analyte in a test sample is provided in which a test sample is contacted with a test strip of a chromatographic bibulous porous material which is capable of moving the test sample laterally along the test strip by capillary migration to ultimate capture by a moiety in a specific capture zone.

Method for detection of non-denatured nucleic acid fragments

Amplification of assay reporters by nucleic acid replication

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Wilmington, DE, United States of America

Richard C Ebersole