Hoffmann-la Roche Inc.

Roche Diagnostics Gmbh

Roche Diagnostic Systems, Inc.

Richard Barner

Josef Hubscher

Max Schmid

Georges Hirth

Paul Hadvary

Jean-Marie Cassal

Klaus Muller

Walter Boguth

Kaspar Burri

Beat Wirz

Reinhard Zell

Walter Huber

Hans G Leuenberger

Josepf Hubscher

Jurg Hurst

Daniel Schlatter

Kasper F Burri

Joseph Hubscher

A biologically recognizing layer on a solid phase consisting of biologically recognizing molecules comprising regions which recognize a substance to be analyzed and regions which do not recognize a substance to be analyzed. The biologically recognizing molecules are aligned on a suitably modified surface by means of molecular regions which do not recognize the substance to be analyzed. The biologically recognizing molecules are cross-linked with the aligning surface and are consequently covalently altered. The molecular regions recognizing the substance to be analyzed are not altered by the covalent bonding and retain their bonding activity. The layer is produced in a novel two-stage method. In the first step, the biologically recognizing molecules, the aligning molecules and carrier molecules are adsorbed. In the second step, the molecules are covalently anchored by cross-linking. Cross-linking is brought about by photolytic activation of a water soluble reagent bonded to the carrier molecules.

Biosensory layers

The invention is concerned with novel heterotrifunctional compounds which contain a chemically reactive group R, a photochemically reactive group Ar--N.sup.3 and a reductively cleavable S--S(X.sub.2)-group. The chemically reactive group and the photochemically reactive group are separated by a spacer group which contains a disulfide group. The water-solubilizing groups are carboxylic acids and sulfonic acids or derivatives thereof which are attached either to the aromatic ring or to the spacer group between the aromatic ring and the cleavable SS group. The compounds facilitate a novel photoaffinity method for the labelling of biomolecules, whereby the labelled biomolecule preserves its water solubility and the labelling molecule preferably projects free into the solution after anchorage to the surface of the biomolecule.

Heterotrifunctional compounds for photoaffinity labelling

Alcohols of the formula ##STR1## wherein R.sup.1 and R.sup.2 are each independently methyl or ethyl or together signify pentamethylene, formed by the enzymatic hydrolysis of a corresponding (RS) alkanoic acid ester are intermediates for Vitamin E.

Dioxolanes

Process for the production of dioxolanes

Glycerine derivatives of the formula ##STR1## wherein the residues R.sup.1, R.sup.2 and R.sup.3 have the significance given in the description, and their hydrates, which inhibit blood platelet activating factor (PAF), are described.

Glycerin derivatives

Novel methyl carbinol derivatives of Vitamin E and a method for their manufacture.

Vitamin E intermediates and a process for their manufacture and

Glycerol derivatives of the formula ##STR1## wherein one of the residues R.sup.1, R.sup.2 and R.sup.3 is a group U of the formula OY.sup.1 or --X.sup.1 --CO--(A.sup.1).sub.n --Z.sup.1, another residue is a group V of the formula OY.sup.2 or --X.sup.2 --CO--(A.sup.2).sub.p --Z.sup.2, and the remaining residue is a group W of the formula --X.sup.3 T--(C.sub.2-6 -alkylene)-N.sup.+ R A.sup.- in which one of X.sup.1, X.sup.2 and X.sup.3 is oxygen or NQ.sup.1 and the other two are oxygen and the remaining symbols have the significance given below, and their hydrates are described and are prepared by introducing or generating the residues R.sup.1, R.sup.2 and R.sup.3 in corresponding glycerol derivatives. The compounds of formula I are active as inhibitors of blood platelet activating factor or as inhibitors of the growth of tumors.

Glycerol derivatives

Novel vitamin E intermediates and a process for their manufacture and

The present invention is directed to a novel process for producing chromane derivatives, which are suitable as intermediates for the manufacture of d-.alpha.-tocopherol (natural vitamin E), and with a process for producing d-.alpha.-tocopherol itself.

Process for synthesizing chromanes

There is described a process for the manufacture of hydroquinone derivatives of the formulae ##STR1## which are valuable intermediates in the synthesis of natural vitamin E.

Hydroquinone derivatives and a process for their preparation

Vitamin E intermediates

Compounds of the general formula ##STR1## wherein R represents an ether protecting group and R.sup.1 is a group of (a)-(d) useful as intermediates.

Growing community of inventors

Witterswil, Switzerland

Richard Barner