Bio-rad Laboratories, Inc.

Siemens Healthcare Diagnostics Gmbh

Bio-red Laboratories, Inc.

Richard B Edwards

Michael I Watkins

Renato B Del Rosario

Suknan S Chang

Patricia A Miranda

Timothy D Knight

Eddy Chapoteau

Wolodymyr Zazulak

Christopher J Macko

Jordan S Honig

Chester Swirski

Provided herein are methods for detecting glycated hemoglobin in, for example, human whole blood, that are not affected by the presence of variation in amino acid sequence that can exist in hemoglobin &#x3b2; chains. The methods detect all glycated hemoglobin in a sample, regardless of the form of the hemoglobin that has been glycated, and thus detect glycated human Hemoglobin A, Hemoglobin S, and Hemoglobin C.

Methods for the detection of glycated hemoglobin

Immunological assays for several biological markers for thyroid disorders in a biological sample are performed in a single test with a combination of sandwich-type, sequential competitive, and serological assays by the use of particles classified into groups that are distinguishable by flow cytometry, one group for the assay of each marker. Each group of particles is coated with a different immunological binding member, and coating densities, co-coating materials, and special buffer solutions are used to adjust for differences in the sensitivities and dynamic ranges of each of the markers in the typical sample.

Multi-analyte diagnostic test for thyroid disorders

Heterogeneous assays for different analytes in a single biological sample are performed simultaneously in a multiplexed assay that combines flow cytometry with the use of magnetic particles as the solid phase and yields an individual result for each analyte. The particles are distinguishable from each other by characteristics that permit them to be differentiated into groups, each group carrying an assay reagent bonded to the particle surface that is distinct from the assay reagents of particles in other groups. The magnetic particles facilitate separation of the solid and liquid phases, permitting the assays to be performed by automated equipment. Assays are also disclosed for the simultaneous detection of antibodies of different classes and a common antigen specificity or of a common class and different antigen specificities. Each type is accomplished by immunological binding at the surfaces of two distinct solid phases in a sequential manner with dissociation of the binding and washing of the solid phase in between the binding steps.

Multiplex flow assays preferably with magnetic particles as solid phase

Multiplex flow assays with magnetic particles as solid phase

Magnetic particles as solid phase for multiplex flow assays

A reagent vessel and support rack for use in an automated clinical analyzer are disclosed. Characteristic features of the vessel are a membrane covering which is puncturable and preferably self-sealing, and characteristic features of the support rack are a rigid wall and retaining members to hold the vessel in the rack with the membrane against the rigid wall of the rack, thereby providing structural support to the membrane, with holes in the wall to permit access to the membrane and hence to the contents of the receptacle.

Reagent receptacle and support rack for automated clinical analyzers

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Vacaville, CA, United States of America

Richard B Edwards