Dekalb Genetics Corporation

Dekalb Plant Genetics

Richard A Yingling

Anthony D Genovesi

This invention relates to the regeneration of fertile monocotyledonous plants from a plant composition that contains microspores. A method of the present invention comprises preculturing a plant composition under a plurality of stressful conditions that promote embryogenesis, isolating microspores at a temperature below about 25.degree. C., culturing the microspores in an embryoid/calli induction medium and regenerating a plant from an embryoid/calli. A solid, porous support system for transferring isolated microspores through a series of subcultures containing different media is also provided. The composition of the media are aspects of the invention.

Isolated microscope and anther culture of maize

This invention relates to the regeneration of fertile monocotyledonous plants from anther or microspore cultures. Fertile corn plants have been regenerated from in vitro cultures by the methods disclosed herein. The invention relates to synergistic effects of pretreatment with a combination of stresses, for example, a carbon structure in the preculture medium capable of inducing embryogenesis such as mannitol at cold temperatures, for example, 10.degree. C. A solid support system has been developed to transfer isolated microspores through a series of subcultures containing different media. The composition of the media are aspects of the invention. Chromosome doubling methods for haploid anther and isolated microspore cultures are presented. This invention also relates to the plants regenerated from microspore or anther cultures, and to their seeds and progeny.

Growing community of inventors

DeKalb, IL, United States of America

Richard A Yingling