Singulex, Inc.

University of California

Becs Technology, Inc.

Novilux, LLC

Richard A Livingston

William E Ortyn

Philippe J Goix

Robert Puskas

Douglas Held

John Allan Todd

Allan H B Wu

Sara Jane Le

Alan Wu

Roger Dean Chamberlain

John Todd

Erich Kyger

Alan H Wu

Brian W Noland

Jeffrey J Bishop

Danni Wang

Michele Gilbert

Luis H Monsalve

John David Jolly

Robert Onorato

The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin. Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.

Highly sensitive system and method for analysis of troponin

The invention encompasses analyzers and analyzer systems that include a single molecule analyzer, methods of using the analyzer and analyzer systems to analyze samples, either for single molecules or for molecular complexes. The single molecule uses electromagnetic radiation that is translated through the sample to detect the presence or absence of a single molecule. The single molecule analyzer provided herein is useful for diagnostics because the analyzer detects single molecules with zero carryover between samples.

Scanning analyzer for single molecule detection and methods of use

The invention provides methods, compositions, kits, and systems for the sensitive detection of cardiac troponin, Such methods, compositions, kits, and systems are useful in diagnosis, prognosis, and determination of methods of treatment in conditions that involve release of cardiac troponin.

Disclosed are methods, kits, and compositions for the highly sensitive detection of molecules. The methods, kits, and compositions are useful in determining concentrations of molecules in samples to levels of 1 femtomolar, 1 attomolar, or lower. The methods, kits, and compositions also allow the determination of concentration over a wide range, e.g., 7-log range, without need for sample dilution.

Methods and compositions for highly sensitive detection of molecules

Cardiovascular disease, e.g., congestive heart failure, is often first diagnosed after the onset of clinical symptoms, eliminating potential for early intervention. The invention provides a multi-marker immunoassay, including cardiac pathology and vascular inflammation biomarkers, yielding a more sensitive assay for early detection of CHF in plasma. A panel consisting of cardiac pathology (cTnI, BNP) and vascular inflammation (IL-6, TNF&#x3b1;, IL-17) biomarkers provided a sensitivity of 94% for association with CHF.

Highly sensitive biomarker panels

The invention encompasses analyzers and analyzer systems that include a single particle analyzer, methods of using the analyzers and analyzers systems to analyze samples, either for single particles, e.g., protein molecules, or for multiple particles (multiplexing), methods of doing business based on the use of the analyzers or analyzer systems of the system, and electronic media for storing parameters useful in the analyzers and analyzer systems of the invention.

Method of analysis for determining a specific protein in blood samples using fluorescence spectrometry

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Webster Graves, MD, United States of America

Richard A Livingston