Hoffmann-la Roche Inc.

Other

Richard A Kaufman

Henry J Rosenfeld

James B Denton

Bruce Charles Farrenkopf

Alexander F Wesolowski

Benjamin A Hahn

John M Konopka

Denise Zaunczkowski

Salvatore Buda

Alex Wesolowski

Janine E Sabo

Diane J Dixon

The invention relates to an improved enzymatic method for determining the concentration of an analyte in a sample body fluid. The improvement comprises a coupled enzyme reaction scheme comprising the in situ generation of reduced nicotinamide coenzyme, with either simultaneous or subsequent re-oxidation of coenzyme by an analyte substrate and substrate specific enzymes. The change in absorbance due to the reoxidation of coenzyme is proportional to the concentration of analyte in the reaction mixture.

In situ nicotinamide coenzyme generating system for enzyme based

A combination absorbent applicator, wipe for teeth and oral applicator comprising a sheath having a closed distal end and an open proximal end for receipt of a fingertip or finger. An abrasive absorbent material pad is on an exterior surface of the sheath adjacent the closed distal end. The abrasive absorbent material pad can be applied to teeth, gums, tongue and lips for cleaning and treatment thereof.

Combination absorbent applicator, wipe for teeth and oral applicator

An enzymatic assay assemblage for ethanol determination is provided. A diamino compound acts as a buffer and a trapping agent for a product of the enzymatic reaction. Long stability of the assay composition is achieved.

Enzymatic Reagents for ethanol assay containing diamino compounds

The present invention provides novel reagent compositions incorporating chlorophosphonazo III and a chelating agent and methods utilizing such compositions for the determination of magnesium and magnesium concurrently with calcium in an analytical sample as well as diagnostic test kits for such determinations.

Method for quantitation of calcium and magnesium and the novel reagent

An enzymatic assay composition for ethanol determination is provided. A compound of the formula

Enzymatic composition for ethanol assay

A reagent for determining quantitatively the amount of phosphorous in bodily fluids which comprises a surface active agent, a strong acid, ammonium molybdate, a heavy metal chelating compound, and anti-oxidant, and deionized water is described. A method of using such a reagent is also described.

Method and reagent for the quantitative determination of phosphorous in

A method for measuring chloride ion concentration in a sample of body fluid, e.g., serum or plasma is described in which a sample blank is made in the same cuvette after the test measurement has been made.

Two reagent system for the colorimetric determination of chloride ions

A method for determining total digoxin levels in a biological fluid sample containing digoxin-protein complex which method comprises disrupting the digoxin-protein complex by treating said sample solution with an effective amount of a dissociation agent selected from one or more members of the group consisting of a C.sub.3 -C.sub.26 saturated or unsaturated fatty acid, a C.sub.2-7 lower alkanol, quinidine and .alpha.-tocopherol so as to release the bound digoxin from serum protein, passing the treated sample solution through a centrifugal ultrafilter so as to selectively pass digoxin containing sample solution through said filter and retaining said serum proteins and determining the digoxin contents in said sample solution in a fluorescence polarization assay. A kit for carrying out the method is provided.

Method and kit for determining total digoxin levels involving agent to

An improved photometric method for the determination of inorganic phosphate in fluids is disclosed. The method makes use of two reagents, an acid reagent and an ammonium molybdate reagent. Utilizing these reagents, the method comprises the following steps:

Photometric method for the determination of inorganic phosphate in

This invention relates to diagnostic reagents and a method for increasing the sensitivity of chemical and enzymatic analysis. In particular, it relates to an improved reagent and method wherein the sensitivity of the analysis is improved by the addition of a water-soluble inclusion compound.

Growing community of inventors

Belleville, NJ, United States of America

Richard A Kaufman