The State University of New York

Richard A Clark

Doris Greiling

Fubao Lin

Dennis K Galanakis

Marcia Simon

James Gailit

Xiang-Dong Ren

Azmin Kahn

Miyoko Kubo

Described herein are fragments of fibronectin and vitronectin and variants thereof that have certain activities, including growth factor-binding activity. Also described are fragments of growth factors that bind to fibronectin and inhibit binding of full-length growth factors to fibronectin. Compositions containing such fragments are useful in cosmetic treatments (e.g., the treatment of wrinkles or UV photodamage of skin), and the treatment of wounds and cancer.

Polypeptides and methods of use

Described herein are fragments of fibronectin and variants thereof that bind growth factors. Compositions containing such a fragment of fibronectin are therefore useful in sequestering growth factors, and complexes containing both a FN fragment and a bound, active growth factor can be used to deliver growth factors to a patient (e.g., to a wound on the patient's skin).

Fibronectin polypeptides and methods of use

Methods and compositions for enhancing fibroblast migration at a wound site are disclosed. The method includes contacting the wound site with fibrinogen that is prepared by a process which includes precipitating plasma with glycine. The compositions includes a lipid rich component and fibrinogen.

Methods and compositions for enhancing fibroblast migration

The invention provides the development of models for cell migration, including an in vitro model and an in vivo model. The in vitro model for cell migration comprises a first extracellular matrix containing a cell (the cell which will migrate) and a second extracellular matrix in physical contact with the first extracellular matrix. The first extracellular matrix simulates a first natural environment in which the cell naturally resides, and the second extracellular matrix simulates a second natural environment into which the cell naturally migrates from the first natural environment. The in vivo model according to the subject invention comprises an animal model having a naturally occurring first extracellular matrix containing a cell, and a second extracellular matrix in physical contact with the first extracellular matrix. The first and second extracellular matrices are generally as described above for the in vitro model, except that the first extracellular matrix is part of an animal model. The primary uses of the models are for screening substances for their effect on cell migration, and for screening extracellular matrices for their effect on cell migration.

Model for cell migration and use thereof

The invention provides a recombinant keratinocyte having nucleic acid encoding a human &bgr;3 integrin subunit introduced therein. This recombinant keratinocyte is able to adhere to fibrin/fibrinogen, and therefore when used in a graft procedure the recombinant keratinocyte is able to adhere to fibrin/fibrinogen present in any fibrin clot in the wound. The invention thus further provides a method of enhancing wound healing in a graft procedure by using the recombinant keratinocytes.

Recombinant keratinocytes

The invention provides an extracellular matrix for wound healing comprising peptides from two or more fibronectin domains in a backbone matrix. In one embodiment, the subject invention provides a hyaluronic acid backbone derivatized with the minimal FN sequences that are optimal for tissue cell recruitment. These constructs can be used to accelerate the healing of acute gaping cutaneous wounds and chronic cutaneous ulcers. The invention thus further provides a method of enhancing wound healing which comprises applying the extracellular matrix to a wound.

Fibronectin peptides-based extracellular matrix for wound healing

The invention provides an extracellular matrix for enhancing wound healing. The extracellular matrix comprises a recombinant fibronectin protein and a backbone matrix, wherein the recombinant fibronectin protein comprises peptides from two or more fibronectin domains. The extracellular matrix facilitates wound healing by providing hemostasis and, in addition, an environment that intrinsically recruits new tissue cells to the wound site. The extracellular matrix according to the subject invention is thus used in a method for enhancing wound healing. The method comprises applying the extracellular matrix to the wound.

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Poquott, NY, United States of America

Richard A Clark