Leica Mikrosysteme Gmbh

Ricardo Vizcaya Benavides

Paul Wurzinger

Rainer Wogritsch

Julia König

A high pressure freezing cartridge () for use in vitrification of a biological sample () is provided, the cartridge () being adapted to fixedly hold a sample chamber () at a sample chamber position () in the cartridge (), a refrigerant channel arrangement comprising at least one refrigerant channel () being provided in the cartridge () and extending from a surface of the cartridge () to the sample chamber position, and the cartridge () comprising a baffle structure () at a baffle position being adapted to interact with a refrigerant stream in the refrigerant channel arrangement before the refrigerant of the refrigerant stream reaches the sample chamber position in the cartridge (). A corresponding method in which the high pressure freezing cartridge () is used is also part of the present disclosure.

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Vienna, Austria

Ricardo Vizcaya Benavides