Stellenbosch University

Lallemand Hungary Liquidity Management LLC

Danstar Ferment Ag

Universiteit Stellenbosch

Riaan Den Haan

John E McBride

Allan C Froehlich

Mark Mellon

Emily Stonehouse

Haowen Xu

Kristen M Deleault

John S Bardsley

Elena E Brevnova

Aaron Argyros

Trisha Barrett

Nicky Caiazza

Charles F Rice

Erin Wiswall

Ryan Skinner

Anne K Warner

Chhayal V Gandhi

Shital A Tripathi

Indraneel Shikhare

Frank Agbogbo

Kevin S Wenger

Yin-Ying Chiu

Jennifer Gosselin

Vineet B Rajgarhia

Heidi H Hau

Merja Elisa Penttila

Vineet Rajgarhia

Merja Penttilä

Alan Belcher

Abigail Foster

Emile Van Zyl

Matti Siika-Aho

Marja Ilmen

Alan Benjamin Gilbert

Sanni Voutilainen

Jaana Uusitalo

Anu Koivula

Danie Lagrange

Deidre Willes

Jim Flatt

Heidi Lau

Elena Brevnova

Naomi Thorngren

Jeff Villari

Abigail S Foster

Chhayal Ghandi

Jeffrey D Keating

Emily A Stonehouse

Heidi Hanson Hau

Dan Murphy

Alan Froehlich

Shaunita Rose

John E Mcbride

Merja Penttila

The present invention is directed to a yeast strain, or strains, secreting a full suite, or any subset of that full suite, of enzymes to hydrolyze corn starch, corn fiber, lignocellulose, (including enzymes that hydrolyze linkages in cellulose, hemicellulose, and between lignin and carbohydrates) and to utilize pentose sugars (xylose and arabinose). The invention is also directed to the set of proteins that are well expressed in yeast for each category of enzymatic activity. The resulting strain, or strains can be used to hydrolyze starch and cellulose simultaneously. The resulting strain, or strains can be also metabolically engineered to produce less glycerol and uptake acetate. The resulting strain, or strains can also be used to produce ethanol from granular starch without liquefaction. The resulting strain, or strains, can be further used to reduce the amount of external enzyme needed to hydrolyze a biomass feedstock during an Simultaneous Saccharification and Fermentation (SSF) process, or to increase the yield of ethanol during SSF at current saccharolytic enzyme loadings. In addition, multiple enzymes of the present invention can be co-expressed in cells of the invention to provide synergistic digestive action on biomass feedstock. In some aspects, host cells expressing different heterologous saccharolytic enzymes can also be co-cultured together and used to produce ethanol from biomass feedstock.

Yeast expressing saccharolytic enzymes for consolidated bioprocessing using starch and cellulose

The present invention provides for heterologous expression of polypeptides encoded by wild-type and condon-optimized variants of cbh1 and/or cbh2 from the fungal organisms(),(),(), and() in host cells, such as the yeast. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

Nucleic acids encoding fungal cellobiohydrolases for expression in yeast

The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized cbh2 genes from the organisms, andsp. in host cells, such as the yeast. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

Heterologous expression of fungal cellobiohydrolase 2 genes in yeast

The present invention is directed to cellulytic host cells. The host cells of the invention expressing heterologous cellulases and are able to produce ethanol from cellulose. According to the invention, host cells expressing a combination of heterologous cellulases can be used to produce ethanol from cellulose. In addition, multiple host cells expressing different heterologous cellulases can be co-cultured together and used to produce ethanol from cellulose. Furthermore, the invention demonstrates for the first time the ability ofto produce ethanol from cellulose. The yeast strains and co-cultures of yeast strains of the invention can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of saccharification and fermentation processes.

Yeast expressing cellulases for simultaneous saccharification and fermentation using cellulose

The present invention provides for heterologous expression of polypeptides encoded by wild-type and codon-optimized variants of cbh1 and/or cbh2 from the fungal organisms(),(),(), and() in host cells, such as the yeast. The expression in such host cells of the corresponding genes, and variants and combinations thereof, result in improved specific activity of the expressed cellobiohydrolases. Thus, such genes and expression systems are useful for efficient and cost-effective consolidated bioprocessing systems.

Host cells, comprisingexpressing heterologous cellulases produce ethanol from cellulose In addition, multiple host cells expressing different heterlogous cellulases can be co-cultured together and used to produce ethanol from cellulose The recombinant yeast strains and co-cultures of the yeast strains can be used to produce ethanol on their own, or can also be used in combination with externally added cellulases to increase the efficiency of sacchanfication and fermentation processes.

Growing community of inventors

Durbanville, South Africa

Riaan Den Haan