University of California

Los Alamos National Security, LLC

Rhiannon L Nolan

Richard A Keller

Hong Cai

Peter M Goodwin

John P Nolan

Bruce E Lehnert

Teresa Ruscetti

Background luminescence is reduced from a solution containing unbound luminescent probes, each having a first molecule that attaches to a target molecule and having an attached luminescent moiety, and luminescent probe/target adducts. Quenching capture reagent molecules are formed that are capable of forming an adduct with the unbound luminescent probes and having an attached quencher material effective to quench luminescence of the luminescent moiety. The quencher material of the capture reagent molecules is added to a solution of the luminescent probe/target adducts and binds in a proximity to the luminescent moiety of the unbound luminescent probes to quench luminescence from the luminescent moiety when the luminescent moiety is exposed to exciting illumination. The quencher capture reagent does not bind to probe molecules that are bound to target molecules and the probe/target adduct emission is not quenched.

Quenching methods for background reduction in luminescence-based probe-target binding assays

A method for making recombinant fluorescent protein standard particles for calibration of fluorescence instruments.

Recombinant fluorescent protein microsphere calibration standard

Growing community of inventors

Santa Fe, NM, United States of America

Rhiannon L Nolan