Transgenomic, Inc.

Edge Biosystems, Inc.

Reyes Candau

Paul D Taylor

Gary Floyd Gerard

John L Seed

Eva Kristyanne

The invention relates to methods for identifying the sequence of one or more variant nucleotides in a nucleic acid molecule. The method involves cleaving a double-stranded nucleic acid molecule containing a mismatch with a mismatch-specific endonuclease which cleaves on the 3' side of the mismatch, and preserving the integrity of the variant nucleotide by ligating a double-stranded linker with a 3&#x2032;-overhang to said variant nucleotide. Because the variant nucleotide is immediately adjacent to the linker, PCR and/or sequence-by-synthesis analysis can be readily carried out.

Method for identifying the sequence of one or more variant nucleotides in a nucleic acid molecule

The invention relates to methods for identifying the sequence of one or more variant nucleotides in nucleic acid molecules. The method involves cleaving a double-stranded nucleic acid molecule containing a mismatch with a mismatch-specific endonuclease which cleaves on the 3' side of the mismatch, and preserving the integrity of the variant nucleotide by ligating a Double-Stranded Linker with a degenerate 3&#x2032;-overhang to said variant nucleotide. Because the variant nucleotide is immediately adjacent to the linker, PCR and/or sequence-by-synthesis analysis can be readily carried out.

A method and materials are described for purification of DNA using ion exchange resins. This ion exchange method provides the means to remove salts and low molecular weight contaminants from DNA in solution without binding or removing the DNA of interest.

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Olney, MD, United States of America

Reyes Candau