University of Minnesota

Recombinetics, Inc.

Reuben S Harris

Scott C Fahrenkrug

William Brown

Stefan R Jonsson

Hiroshi Matsuo

Brett Anderson

Michael Carpenter

Rebecca St Claire Larue

Amber Renee St Martin

Hideki Aihara

Daniel James Salamango

Emily Law

Rebecca St Larue

Real-time systems for monitoring base editing in living cells, including base editing by APOBEC-Cas9 fusions, is provided herein.

Real-time reporter systems for monitoring base editing

Materials and methods for using modified Cas9-APOBEC fusion polypeptides for targeted modification of specific DNA sequences are provided herein.

Site-specific DNA base editing using modified APOBEC enzymes

Hybridoma cell lines produce monoclonal antibodies that specifically bind to an APOBEC3 protein. The antibodies can be used in various methods. In some aspects, an anti-APOBEC3 antibody may be immobilized to a substrate. In another aspect, this disclosure provides a vector that includes a nucleic acid sequence encoding antibody produced by a hybridoma cell line that produces an antibody that specifically binds to an APOBEC3 protein.

Anti-APOBEC3 antibodies and methods of making and using

The present disclosure describes an apparatus including a waste compartment, a membrane compartment on the waste compartment, and a wash buffer reservoir on the membrane compartment. The membrane compartment includes a membrane compartment outlet aperture and is configured to hold at least one membrane, and the membrane compartment outlet aperture fluidly connects the membrane compartment and the waste compartment. The wash buffer reservoir comprises a wash buffer reservoir outlet aperture, and the wash buffer reservoir outlet aperture fluidly connects the wash buffer reservoir and the membrane compartment. The apparatus may be used to wash membranes used in analytic techniques, such as membranes for gel electrophoresis.

Membrane washing apparatus

Transgenic pigs that express one or more non-porcine cytosine deaminases are described as well as methods of making and using such pigs.

Using cytosine deaminases to diminish retroelement transfer from pigs to humans

The document provides modified cytosine deaminases with increased solubility and high levels of DNA cytosine deaminase activity.

Growing community of inventors

Saint Paul, MN, United States of America

Reuben S Harris