University of Zürich

Universitè De Genève

University of Pretoria

University of Geneva

Les Hôpitaux Universitaires De Genève

Renier Myburgh

Karl-Heinz Krause

Patrick Salmon

Roberto F Speck

Francis Rousset

Tafadzwa Mlambo

Marco Alessandrini

Simon Bredl

The present disclosure relates to miRNA expression constructs, such as for expression of multiple miRNAs and use thereof to knockdown target gene expression. In some aspects, the expression constructs include a promoter element, a spacer sequence and a miRNA coding sequence. In some aspects, constructs provide enhanced immune cell function.

Micro RNA expression constructs and uses thereof

Recombinant nucleic acid molecules are provided that form hair pin structures and can be used to down-regulate gene expression. For example, a nucleic acid molecule can comprise a flanking and lower stem loop sequence from a mir-16 gene; an antisense target sequence; a mir-30 loop sequence; a complement of the anti-sense target sequence; and a lower stem loop complementary to the mir-16 sequence. Methods for down regulating gene expression in a cell using such recombinant nucleic acid molecules are also provided.

Nucleic acids for down-regulation of gene expression

Growing community of inventors

Pretoria, South Africa

Renier Myburgh