Pfizer Corporation

Renee L Procopio-melino

Amarnauth Shastrie Prashad

Xiaotian Zhong

Jose Manuel Gomes

Kaushik Dutta

Frank W Kotch

Vimalkumar B Patel

Optimizing production of selectively capped, and uncapped, cysteines on antibodies by manipulation of cell growth conditions including the deliberate depletion of cysteine and/or cystine in the cell culture process by way of media components, batch duration, or cell density to achieve efficient production of proteins including antibody-drug-conjugates (ADCs).; conjugating a TNB-capped cysteine-containing protein by reacting it with a reducing agent capable of detaching the TNB-capping moieties from the protein without significantly reducing antibody inter-chain sulfur bonds, and conjugating reduced sulfur bonds on the protein to a payload through a reactive linking moiety.

Growing community of inventors

Andover, MA, United States of America

Renee L Procopio-melino