Rutgers, the State University of New Jersey

Rene S Schloss

Martin L Yarmush

Tim Maguire

Nir I Nativ

Gabriel A Yarmush

Francois Berthiaume

Mehdi Ghodbane

Eric J Wallenstein

Jean-Pierre Dolle

Anil Shrirao

Bhaskar Mitra

Ileana Marrero-Berrios

Rohit Jindal

Described herein is a cell culture device and methods of use in three-dimensional cell co-cultures and for use in studying paracrine signaling in vitro.

Multilayer stackable tissue culture platform for 3D co-culture

This application discloses alginate microencapsulation-mediated differentiation of embryonic stem cells and use of the stem cell differentiation method for the development of effective treatment of various diseases and disorders. The microencapsulation of embryonic stem (ES) cells results in decreased cell aggregation and enhanced neural lineage differentiation through incorporating the soluble inducer retinoic acid (RA) into the permeable microcapsule system. This differentiation process can be augmented by differentiation pathway regulators such as PPAR agonists.

Lineage differentiation of encapsulated embryonic stem cells

The present invention relates to a system and methods for identifying a compound for de-fatting and functional recovery of macrosteatotic hepatocytes.

In vitro model of macrosteatotic (fatty) liver

This invention discloses devices and methods for high throughput skin sensitization detection. The devices comprise a microfabricated chamber comprising a region having one or more input channels and an outlet, and a face suitable for mounting a skin tissue and in fluidic communication with the region. The devices can be used in the methods for determining a prognosis of sensitization in an animal subject and identifying compounds that do not cause sensitization and thus are suitable for preparing cosmetic compositions.

High throughput sensitization detection devices and methods

The present invention provides systems and methods for improving the efficiency of a transient gene delivery system to differentiating embryonic stem (ES) cells by serum starving the targeted cells for one to three days prior to transfection. Such a serum starvation surprisingly resulted in increased expression of a constitutively-controlled plasmid from 50.4% to 83.2% of the population and increased expression of a promoter/enhancer controlled plasmid from &#x2dc;1.4% to &#x2dc;3.7% of the population.

Media conditioning for improving gene delivery efficiency to differentiating embryonic stem cells

Alginate polyelectrolyte encapsulation is used for the controlled differentiation of embryonic stem cells. An isolated cell population is provided. The cell population includes a single cell suspension of ES cells encapsulated within an alginate polyelectrolyte microenvironment. The encapsulated ES cells are capable of differentiating within said microenvironment into hepatocyte lineage cells in the absence of embryoid body intermediates or growth factor supplementation.

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East Brunswick, NJ, United States of America

Rene S Schloss