Bayer Cropscience Nv

Bayer Bioscience N.v.

Bayer Cropscience Ag

Basf Agricultural Solutions Seed US LLC

Rene Ruiter

Bernd Laber

Kathleen D'Halluin

Manuel Dubald

Gerhard Johann

Anne Rolland

Kathleen D'halluin

Chantal Vanderstraeten

Michiel Van Lookeren Campagne

Kathleen D′Halluin

Rüdiger Hain

Michiel Van Lookeren-Campagne

Methods and means are provided for the exact exchange in eukaryotic cells, such as plant cells, of a target DNA sequence for a DNA sequence of interest through homologous recombination, whereby the selectable or screenable marker used during the homologous recombination phase for temporal selection of the gene replacement events can subsequently be removed without leaving a foot-print employing a method for the removal of a selected DNA flanked by two nucleotide sequences in direct repeats.

Methods and means for exact replacement of target DNA in eukaryotic organisms

Methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving 'double stranded break' inducing enzymes. More specifically, the methods involve introducing the DNA that is to be inserted into the induced break into the plant cell via direct DNA delivery and incubating the plant cells in a plant phenolic compound prior to DNA delivery. Also provided are improved I-SceI encoding nucleotide sequences having a CG content of 50-60%.

Targeted DNA insertion in plants

Alternative and/or improved methods are described for the exact removal of a selected subfragment from a DNA molecule by intrachromosomal recombination between two directly repeated DNA sequences using a rare-cleaving double stranded break inducing DNA endonuclease expressed under control of a micro-spore specific promoter. These methods can be applied for the exact exchange of a target DNA fragment for a DNA fragment of interest in plant cells and plants.

Methods and means for removal of a selected DNA sequence

A method is described for the exact removal of a selected subfragment from a DNA molecule by intrachromosomal recombination between two directly repeated DNA sequences using a rare-cleaving double stranded break inducing DNA endonuclease expressed under control of a micro-spore specific promoter. This method can be applied in a method for the exact exchange of a target DNA fragment for a DNA fragment of interest in plant cells and plants.

Methods and means are provided to improve targeted DNA insertion in plants using rare-cleaving 'double stranded break' inducing enzymes. Also provided are improved I-SceI encoding nucleotide sequences.

Growing community of inventors

Heusden, Belgium

Rene Ruiter