Sartorius Stedim Biotech Gmbh

Bavarian Nordic A/s

Otto-von-guericke-universität

Rene Faber

Wolfgang Demmer

Udo Reichl

Sara Post Hansen

Michael Wolff

Anders Peter Gram

Hans-Heinrich Hoerl

Martin Leuthold

Axel Thiefes

Christian Brumm

The present invention relates to a method for separating viruses from a contaminant-containing liquid medium using two adsorbents having cationic groups, wherein the viruses are adsorbed to the first adsorbent and subsequently eluted and wherein the contaminants present in the resulting eluate are subsequently adsorbed to the second adsorbent. The yield and purity of the viruses obtained as per the method according to the invention is increased by the addition of multivalent anions during the adsorption of the contaminants to the second adsorbent.

Method for separating viruses from a contaminant-containing liquid

A porous, non-particulate, convectively permeable polysaccharide matrix has a surface on which there is fixed a grafted-on polymer derived from at least one ethylenic monomer compound having functional groups, wherein the polysaccharide matrix is prepared by grafting a porous, non-particulate, convectively permeable polysaccharide starting matrix with the at least one ethylenic monomer compound in the presence of an organic acid having at least one carboxylic acid group and/or at least one acidic XH group, where X=&#x2014;O, &#x2014;S, or &#x2014;N, and of a transition metal or lanthanide compound. The polysaccharide matrix has a high protein binding capacity. A process for preparing the polysaccharide matrix and a method for using the polysaccharide matrix for material separation also are provided.

Polysaccharide matrix having a grafted polymer, method for producing the same and use thereof

The present invention relates to methods for purification of Vaccinia viruses (VV) and/or Vaccinia virus (VV) particles, which can lead to highly pure and stable virus preparations of predominantly biologically active viruses. The invention encompasses purifying a virus preparation in a sterilized way with high efficiency and desirable yield in terms of purity, biological activity and stability, aspects advantageous for industrial production.

Purification of vaccinia viruses using hydrophobic interaction chromatography

The present invention relates to a method for the validation of a non-particulate ion exchange adsorber and a kit for the validation of a non-particulate ion exchange adsorber.

Method for qualifying a non-particulate ion-exchanger adsorber

The present invention relates to a method of isolating phenolic secondary plant constituents from plant material using a microporous membrane which displays affinity ligands for the phenolic secondary plant constituents.

Method of obtaining secondary plant constituents

Membrane holder for membrane adsorber chromatography comprising a housing body () with a reception space for at least one layer of adsorber membranes () and at least one first housing cap () for closing a first reception space opening, which can be fixed to the housing body (), a liquid stream being introducible via first liquid guide means () through the first housing cap () and the first reception opening into the reception opening, so that it passes through the adsorber membranes () and flows away through a second reception space opening. The first housing cap () can be screwed as a first union nut onto the housing body () and a first brace element (), which can be clamped between the first cap () and the housing body () via a flange () extending beyond the first reception space opening, can be fixed leaktightly in a basket-like insert () with a liquid-tight wall and a perforated bottom (), which is used to receive the adsorber membranes (). The outer contour of the basket-like insert () being fitted in a leaktight fashion onto an inner contour of the reception space.

Growing community of inventors

Goettingen, Germany

Rene Faber